Global analysis of the AP2/ERF gene family in rose (Rosa chinensis) genome unveils the role of RcERF099 in Botrytis resistance.
Global analysis of the AP2/ERF gene family in rose (Rosa chinensis) genome unveils the role of RcERF099 in Botrytis resistance.
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对玫瑰（Rosa chinensis）基因组中 AP2/ERF 基因家族的整体分析揭示了 RcERF099 在贵腐菌抗性中的作用
DOI：
10.1186/s12870-020-02740-6
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发表时间：
2020-11-23
影响因子：
5.3
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Li D;Liu X;Shu L;Zhang H;Zhang S;Song Y;Zhang Z
AP 2/ERFs是植物中一个大的转录因子家族。AP 2/ERF基因家族被认为是参与植物生物和非生物胁迫应答的关键基因，但目前还没有对蔷薇属植物AP 2/ERF基因家族进行全面的研究，世界上最重要的观赏作物。本研究对蔷薇属植物的AP 2/ERF家族基因（RcERFs）进行了全基因组分析，包括鉴定、基因结构、系统发育关系、染色体定位、共线性分析以及表达模式等。在整个系统发育分析中，共131个AP 2/ERF基因在玫瑰基因组中分为5个亚群。RcERF分布在玫瑰的所有七条染色体上，基因组复制可能在其复制中发挥了关键作用。此外，Ka/Ks分析表明，重复的RcERF基因往往经过纯化选择有限的功能分化。基因表达分析表明，23个RcERFs诱导的坏死营养型真菌病原体灰葡萄孢的感染。推测这些RcERFs是与玫瑰抗灰葡萄孢侵染反应的候选基因。通过病毒诱导的基因沉默，我们证实了RcERF 099是参与玫瑰花瓣抗B.cinerea的重要调控因子。总之，我们的研究结果表明，有必要对玫瑰中的AP 2/ERF基因家族进行进一步的研究，并促进其在改善遭受生物胁迫的玫瑰中的潜在应用。在线版本包含补充材料，可通过10.1186/s12870-020-02740-6获得。
The AP2/ERFs belong to a large family of transcription factors in plants. The AP2/ERF gene family has been identified as a key player involved in both biotic and abiotic stress responses in plants, however, no comprehensive study has yet been carried out on the AP2/ERF gene family in rose (Rosa sp.), the most important ornamental crop worldwide. The present study comprises a genome-wide analysis of the AP2/ERF family genes (RcERFs) in the rose, involving their identification, gene structure, phylogenetic relationship, chromosome localization, collinearity analysis, as well as their expression patterns. Throughout the phylogenetic analysis, a total of 131 AP2/ERF genes in the rose genome were divided into 5 subgroups. The RcERFs are distributed over all the seven chromosomes of the rose, and genome duplication may have played a key role in their duplication. Furthermore, Ka/Ks analysis indicated that the duplicated RcERF genes often undergo purification selection with limited functional differentiation. Gene expression analysis revealed that 23 RcERFs were induced by infection of the necrotrophic fungal pathogen Botrytis cinerea. Presumably, these RcERFs are candidate genes which can react to the rose’s resistance against Botrytis cinerea infection. By using virus-induced gene silencing, we confirmed that RcERF099 is an important regulator involved in the B.cinerea resistance in the rose petal. Overall, our results conclude the necessity for further study of the AP2/ERF gene family in rose, and promote their potential application in improving the rose when subjected to biological stress. The online version contains supplementary material available at 10.1186/s12870-020-02740-6.
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