Expression of collagenase (MMP2), stromelysin (MMP3) and tissue inhibitor of the metalloproteinases (TIMP1) in pancreatic and ampullary disease.
Expression of collagenase (MMP2), stromelysin (MMP3) and tissue inhibitor of the metalloproteinases (TIMP1) in pancreatic and ampullary disease.
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DOI：
10.1038/bjc.1996.190
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发表时间：
1996-04
影响因子：
8.8
通讯作者：
Neoptolemos, JP
中科院分区：
文献类型：
--
作者：
Bramhall, SR;Stamp, GWH;Dunn, J;Lemoine, NR;Neoptolemos, JP
现在人们认识到，上皮-间质的相互作用在包括肿瘤和炎症在内的广泛的疾病过程中是重要的。金属蛋白酶是基质降解和重塑的中心，是肿瘤侵袭和转移的关键事件，也可能参与慢性炎症中发生的组织变化。应用抗胶原酶(MMP2)、基质分解酶(MMP3)和金属蛋白酶组织抑制因子(TIMP1)的抗体，对50例胰腺癌(n=27)、壶腹癌(n=12)、下胆管癌(n=3)、神经内分泌肿瘤(n=3)和慢性胰腺炎(n=5)进行免疫组织化学染色。胰腺癌和壶腹癌中MMP2、MMP3和TIMP1值均高于其他病理组织，且胰腺癌和壶腹癌中恶性上皮细胞的免疫反应性高于间质组织(胰腺癌中MMP2100%比37%，MMP393%比15%，TIMP193%比4%，P<；0.0001)。两种抗体对MMP2的免疫反应性(P<；0.0001)、MMP2与TIMP_1的免疫反应性(P<；0.0001)、MMP3与TIMP_1的免疫反应性(P<；0.0001)之间有很强的相关性。TIMP1在有淋巴结转移的胰腺癌和壶腹癌中的表达明显低于无淋巴结转移的胰腺癌(P<；0.02)，且MMP2的表达增强与肿瘤分化程度有关(P<；0.01)。结果提示，MMP2、MMP3和TIMP1与胰腺癌和壶腹癌的侵袭表型有关。
It is now recognised that epithelial-stromal interactions are important in a wide range of disease processes including neoplasia and inflammation. Metalloproteinases are central to matrix degradation and remodelling, which are key events in tumour invasion and metastasis and may also be involved in tissue changes occurring in chronic inflammation. Immunohistochemistry was performed on sections from 50 patients with pancreatic cancer (n = 27), ampullary cancer (n = 12), low bile duct cancer (n = 3), neuroendocrine tumours (n = 3) and chronic pancreatitis (n = 5), using antibodies raised against collagenase (MMP2), stromelysin (MMP3) and tissue inhibitor of metalloproteinase (TIMP1) and developed using the avidin-biotin complex method. Abundance of MMP2, MMP3 and TIMP1 was greater in pancreatic and ampullary cancer than any other pathology and immunoreactivity in the malignant epithelial cells in pancreatic and ampullary cancer was greater than in the stromal tissues (in pancreatic cancer: MMP2 100% vs 37%, MMP3 93% vs 15%, TIMP1 93% vs 4%, P < 0.0001). There were strong correlations between the immunoreactivity of the two antibodies for MMP2 (P < 0.0001), between MMP2 and TIMP1 (P < 0.0001) and between MMP3 and TIMP1 (P < 0.0001). The immunoreactivity for TIMP1 in pancreatic and ampullary cancers with lymph node metastases was significantly less compared with those cases without lymph node metastases (P < 0.02) and there was an association between increased immunoreactivity for MMP2 and the degree of tumour differentiation (P < 0.01). The results implicate MMP2, MMP3 and TIMP1 in the invasive phenotype of pancreatic and ampullary cancer.
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影响因子：
9.6
作者：
HADDOCK, G;CARTER, DC
通讯作者：
CARTER, DC
影响因子：
3.1
作者：
MATRISIAN, LM;MCDONNELL, S;HENDRIX, MJC
通讯作者：
HENDRIX, MJC
DOI：
10.1016/0006-291x(82)92042-3
发表时间：
1982-01-01
影响因子：
3.1
作者：
BISWAS, C
通讯作者：
BISWAS, C
影响因子：
3.3
作者：
CHARPIN, C;LISSITZKY, JC;TOGA, M
通讯作者：
TOGA, M
影响因子：
6.4
作者：
FORSTER, SJ;TALBOT, IC;CRITCHLEY, DR
通讯作者：
CRITCHLEY, DR