FoxD3 regulates cranial neural crest EMT via downregulation of tetraspanin18 independent of its functions during neural crest formation.
FoxD3 regulates cranial neural crest EMT via downregulation of tetraspanin18 independent of its functions during neural crest formation.
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DOI：
10.1016/j.mod.2014.02.004
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发表时间：
2014-05
影响因子：
2.6
通讯作者：
Gammill, Laura S.
中科院分区：
文献类型：
--
作者：
Fairchild, Corinne L.;Conway, Joseph P.;Schiffmacher, Andrew T.;Taneyhill, Lisa A.;Gammill, Laura S.
The scaffolding protein tetraspanin18 (Tspan18) maintains epithelial cadherin-6B (Cad6B) to antagonize chick cranial neural crest epithelial-to-mesenchymal transition (EMT). For migration to take place, Tspan18 must be downregulated. Here, we characterize the role of the winged-helix transcription factor FoxD3 in the control of Tspan18 expression. Although we previously found that Tspan18 mRNA persists several hours past the stage it would normally be downregulated in FoxD3-deficient neural folds, we now show that Tspan18 expression eventually declines. This indicates that while FoxD3 is crucial for initial downregulation of Tspan18, other factors subsequently impact Tspan18 expression. Remarkably, the classical EMT transcription factor Snail2 is not one of these factors. As in other vertebrates, FoxD3 is required for chick cranial neural crest specification and migration, however, FoxD3 has surprisingly little impact on chick cranial neural crest cell survival. Strikingly, Tspan18 knockdown rescues FoxD3-dependent neural crest migration defects, although neural crest specification is still deficient. This indicates that FoxD3 promotes cranial neural crest EMT by eliciting Tspan18 downregulation separable from its Tspan18-independent activity during neural crest specification and survival.
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DOI：
10.1083/jcb.201008173
发表时间：
2011-06-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Oda H;Takeichi M
通讯作者：
Takeichi M
影响因子：
2.5
作者：
Khudyakov, Jane;Bronner-Fraser, Marianne
通讯作者：
Bronner-Fraser, Marianne
影响因子：
2.5
作者：
Montero-Balaguer, Mercedes;Lang, Michael R.;Knapik, Ela W.
通讯作者：
Knapik, Ela W.
影响因子：
7.2
作者：
Meng, Wenxiang;Takeichi, Masatoshi
通讯作者：
Takeichi, Masatoshi
影响因子：
4.6
作者：
Nitzan, Erez;Krispin, Shlomo;Kalcheim, Chaya
通讯作者：
Kalcheim, Chaya