Characteristics of the Intestinal Microorganisms in Middle-Aged and Elderly Patients: Effects of Smoking.
Characteristics of the Intestinal Microorganisms in Middle-Aged and Elderly Patients: Effects of Smoking.
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吸烟对中老年患者肠道微生物的影响
DOI：
10.1021/acsomega.1c02120
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发表时间：
2022-01-18
期刊：
影响因子：
4.1
通讯作者：
Xie X
中科院分区：
文献类型：
--
作者：
Yang HT;Xiu WJ;Liu JK;Yang Y;Zhang YJ;Zheng YY;Wu TT;Hou XG;Wu CX;Ma YT;Xie X
吸烟影响许多疾病的发生和发展。本研究旨在探讨中老年人肠道植物群的结构及吸烟对肠道植物群的影响。研究方法：我们收集了188例中老年男性患者的人群信息、生化指标和患者粪便，并对其粪便进行了肠道植物群16 S rRNA基因检测。结果如下：我们对纳入人群的肠道结构进行了聚类分析，发现各组之间的吸烟人数存在显著差异（p = 0.011）。随后，对目前吸烟者和非吸烟者进行了微生物多样性分析。结果表明，两组之间的物种组成有显着差异（p = 0.029）。通过对LEfSe鉴别菌的分析发现，目前吸烟患者中双歧杆菌属和粪杆菌属的丰度较低，而丹毒丝菌科水平下志贺菌属、副雷沃菌属、伯克霍尔德菌属、苏特氏菌属、巨单胞菌属和p-75-a5的丰度稍高。我们分析了这八种不同细菌的丰度与临床指标之间的相关性。结果表明：双歧杆菌的丰度与空腹血糖呈负相关（r =-0.198，p = 0.006），与尿酸呈正相关（r = 0.207，p = 0.004）和总胆红素（r = 0.175，p = 0.017）;志贺菌与空腹血糖呈正相关（r = 0.160，p = 0.028）和尿酸（r = 0.153，p = 0.036）水平;副孢菌属和BMI（r =-0.172，p = 0.018）呈负相关;伯克霍尔德菌属的丰度与γ-谷氨酰转移酶呈正相关（r = 0.146，p = 0.045）水平; Sutterella与空腹血糖相关（r = 0.143，p = 0.05）和肌酐水平（r =-0.16，p = 0.027），与空腹血糖呈正相关，与肌酐呈负相关。结论：在中老年心血管疾病患者中，吸烟可使双歧杆菌的丰度降低，而某些阴性菌如伯克霍尔德菌、苏特氏菌、巨单胞菌的丰度增加。
Introduction: Smoking affects the occurrence and development of many diseases. We attempt to study the structure of intestinal flora in the middle-aged and elderly population as well as how smoking affects the intestinal flora. Methods: We collected population information, biochemical indicators, and patient feces from 188 middle-aged and elderly male patients, and their feces were tested for the 16S rRNA gene of intestinal flora. Results: We performed a cluster analysis on the intestinal structure of the included population and found that there was a significant difference in the number of smokers between each group (p = 0.011). Subsequently, the microbiological diversity analysis of current smokers and nonsmokers was carried out. The results indicated that there was a significant difference in species composition between the two groups (p = 0.029). Through the analysis on LEfSe differential bacteria, it was found that in current smoking patients, the abundances of the genus Bifidobacterium and the genus Coprobacillus were less, while the abundances of the genera Shigella, Paraprevotella, Burkholderia, Sutterella, Megamonas, and p-75-a5 under the family level of Erysipelotrichaceae were slightly high. We analyzed the correlation between the abundances of these eight different bacteria and clinical indicators. The results revealed the following: the abundance of the genus Bifidobacterium was negatively correlated with fasting blood glucose (r = −0.198, p = 0.006) and positively correlated with uric acid (r = 0.207, p = 0.004) and total bilirubin (r = 0.175, p = 0.017); Shigella bacteria were positively correlated with fasting blood glucose (r = 0.160, p = 0.028) and uric acid (r = 0.153, p = 0.036) levels; the genus Paraprevotella and BMI (r = −0.172, p = 0.018) are negatively correlated; the abundance of the genus Burkholderia was positively correlated with γ-glutamyltransferase (r = 0.146, p = 0.045) levels; Sutterella was correlated with fasting blood glucose (r = 0.143, p = 0.05) and creatinine level (r = −0.16, p = 0.027), which was positively correlated with fasting blood glucose and negatively correlated with creatinine. Conclusions: In middle-aged and elderly patients with cardiovascular disease, smoking can reduce the abundance of Bifidobacterium, while the abundances of some negative bacteria such as Burkholderia, Sutterella, and Megamonas increase.
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影响因子：
5.7
作者：
Balaguer F;Enrique M;Llopis S;Barrena M;Navarro V;Álvarez B;Chenoll E;Ramón D;Tortajada M;Martorell P
通讯作者：
Martorell P
影响因子：
64.8
作者：
通讯作者：
--
玉米赤霉烯酮改变断奶兔盲肠微生物群的多样性和组成
影响因子：
--
作者：
Li P;Yang S;Zhang X;Huang S;Wang N;Wang M;Long M;He J
通讯作者：
He J
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
4.4
作者：
Emoto, Takuo;Yamashita, Tomoya;Hirata, Ken-ichi
通讯作者：
Hirata, Ken-ichi