Altered Rheological Properties of Saliva with Aging in Mouse Sublingual Gland
Altered Rheological Properties of Saliva with Aging in Mouse Sublingual Gland
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小鼠舌下腺随衰老唾液流变特性的改变
DOI：
10.1177/00220345221076071
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发表时间：
2022
影响因子：
7.6
通讯作者：
Hosokawa R.
中科院分区：
文献类型：
--
作者：
Yamada M.;Masaki C.;Mukaibo T.;Munemasa T.;Nodai T.;Kondo Y.;Hosokawa R.
唾液中的粘蛋白通过保留水分子在口腔粘膜的水合和润滑中起关键作用，并且其功能受损可能与非唾液过少依赖性口干症相关。由于衰老是一个复杂的多因素过程，因此对唾液腺功能和分泌唾液的流变学性质的唾液依赖性影响尚未完全了解。我们的目的是评估与年龄相关的变化，唾液的流变学特性，并阐明其潜在的机制。我们进行了离体下颌下腺（SMG）和舌下腺（SLG）灌注实验，从年轻（12周龄）和老年（27月龄）雌性C57 BL/6 J小鼠的离体腺体收集唾液，并通过测定spinnbarkeit（粘弹性）来研究流变学特性。虽然两种小鼠的SMG和SLG中的液体分泌相当，但spinnbarkeit显示老年小鼠的SLG唾液比年轻小鼠显著减少。青年和老年SLG唾液中GalNAc浓度无显著差异。SLG唾液的液相色谱/串联质谱分析显示，（Hex）1（HexNAc）1（NeuAc）1atm/z793.31是两种小鼠中通常检测到的SLG唾液中最丰富的O-聚糖结构。唾液腺组织凝集素染色显示SLG与怀槐凝集素Ⅱ（MAL Ⅱ）强染色，而接骨木凝集素（SNA）染色较弱。Western blot分析证实，编码α-2，3唾液酸唾液酸转移酶SIAT 4-A的St 3gal 1的mRNA表达在老年小鼠的SLG中显示出减少。SLG唾液中凝集素印迹分析显示，MAL II检测到的相对信号强度在老年SLG中显着降低。我们的研究结果表明，衰老小鼠SLG中spinnbarkeit减少是由于与α-2，3唾液酸唾液酸转移酶表达相关的唾液酸下调。
Mucin in saliva plays a critical role in the hydration and lubrication of the oral mucosa by retaining water molecules, and its impaired function may be associated with hyposalivation-independent xerostomia. Age-dependent effects on salivary gland function and rheological properties of secreted saliva are not fully understood as aging is a complex and multifactorial process. We aimed to evaluate age-related changes in the rheological properties of saliva and elucidate the underlying mechanism. We performed ex vivo submandibular gland (SMG) and sublingual gland (SLG) perfusion experiments to collect saliva from isolated glands of young (12 wk old) and aged (27 mo old) female C57BL/6J mice and investigate the rheological properties by determining the spinnbarkeit (viscoelasticity). While fluid secretion was comparable in SMG and SLG of both mice, spinnbarkeit showed a significant decrease in SLG saliva of aged mice than that of young mice. There were no significant differences in GalNAc concentration between young and aged SLG saliva. Liquid chromatography/tandem mass spectrometry analysis of SLG saliva revealed that (Hex)1(HexNAc)1(NeuAc)1atm/z793.31 was the most abundant O-glycan structure in SLG saliva commonly detected in both mice. Lectin staining of salivary gland tissue showed that SLG stained strongly withMaackia amurensislectin II (MAL II) whileSambucus nigraagglutinin (SNA) stained little, if any, SLG. The messenger RNA expression ofSt3gal1 that encodes an α-2,3 sialic acid sialyltransferase SIAT4-A showed a decrease in SLG of aged mice, confirmed by a Western blot analysis. Lectin blot analysis in SLG saliva revealed that the relative signal intensity detected by MAL II was significantly lower in aged SLG. Our results suggest that spinnbarkeit decreases in SLG of aging mice due to downregulation of sialic acid linked to α-2,3 sialic acid sialyltransferase expression.
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影响因子：
3.8
作者：
Osailan, S.;Pramanik, R.;Proctor, G. B.
通讯作者：
Proctor, G. B.
影响因子：
4.6
作者：
Gao, Xin;Oei, Maria S.;Melvin, James E.
通讯作者：
Melvin, James E.
人类全唾液粘度与唾液酸含量之间的相关性。
DOI：
10.2329/perio.22.366
发表时间：
1980
期刊：
Aichi-Gakuin dental science
影响因子：
--
作者：
F. Ito;S. Yamada;Y. Mizuno;N. Sugihara;L. S. Chen
通讯作者：
L. S. Chen
DOI：
10.1016/0006-3002(61)90649-7
发表时间：
1961
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
A. Gottschalk;M. A. Thomas
通讯作者：
M. A. Thomas
DOI：
10.1016/j.bbrc.2019.03.144
发表时间：
2019-05-21
影响因子：
3.1
作者：
Kameyama, Akihiko;Tin, Wai Wai Thet;Sakaguchi, Midori
通讯作者：
Sakaguchi, Midori