Francisella tularensis novicida infection competence differs in cell lines derived from United States populations of Dermacentor andersoni and Ixodes scapularis.
Francisella tularensis novicida infection competence differs in cell lines derived from United States populations of Dermacentor andersoni and Ixodes scapularis.
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DOI：
10.1038/s41598-018-30419-4
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发表时间：
2018-08-23
影响因子：
4.6
通讯作者：
Noh SM
中科院分区：
文献类型：
--
作者：
Reif KE;Ujczo JK;Alperin DC;Noh SM
在美国，虽然病原体和蜱的地理分布和宿主范围重叠，但革蜱属是土拉弗朗西斯菌亚种（ssp.）的常见媒介，而肩胛骨伊蚊则不是。为了检验细胞水平上的感染能力差异是否支持这些生态差异，我们评估了安德氏弧菌（DAE100）和镰形弧菌（ISE6）细胞系对土拉菌的感染能力。novicida （F. novicida）感染。重要的是，安德氏弓形虫是土拉菌属和新弓形菌的载体。我们假设新乳杆菌感染在安德氏乳杆菌中比在肩胛乳杆菌细胞中更多产。具体来说，我们确定了在DAE100和ISE6细胞之间是否存在新生f.s icicida i)入侵，ii)复制或iii)蜱虫细胞活力的差异。我们进一步研究了温度对感染动力学的影响。两种细胞系均对新乳杆菌感染具有容纳性；然而，与ISE6细胞相比，DAE100细胞的细菌水平和死亡率明显更高。环境温度下的感染延长了细菌维持在高水平的时间，并降低了两种细胞系的蜱细胞死亡率。确定媒介能力的细胞决定因素对于理解蜱传疾病生态学和设计有效的干预策略至关重要。
In the United States, Dermacentor spp. are common vectors of Francisella tularensis subspecies (ssp.), while Ixodes scapularis is not, though the geographic distribution and host range of pathogen and tick overlap. To examine if differences in infection competence at the cellular level underpin these ecological differences, we evaluated the competence of D. andersoni (DAE100) and I. scapularis (ISE6) cell lines to support F. tularensis ssp. novicida (F. novicida) infection. Importantly, D. andersoni is a vector for both F. tularensis spp. tularensis, and F. novicida. We hypothesized F. novicida infection would be more productive in D. andersoni than in I. scapularis cells. Specifically, we determined if there are differences in F. novicida i) invasion, ii) replication, or iii) tick cell viability between DAE100 and ISE6 cells. We further examined the influence of temperature on infection kinetics. Both cell lines were permissive to F. novicida infection; however, there were significantly higher bacterial levels and mortality in DAE100 compared to ISE6 cells. Infection at environmental temperatures prolonged the time bacteria were maintained at high levels and reduced tick cell mortality in both cell lines. Identifying cellular determinants of vector competence is essential in understanding tick-borne disease ecology and designing effective intervention strategies.
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影响因子：
6.7
作者：
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