Genome-wide screen for temperature-regulated genes of the obligate intracellular bacterium, Rickettsia typhi.
Genome-wide screen for temperature-regulated genes of the obligate intracellular bacterium, Rickettsia typhi.
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DOI：
10.1186/1471-2180-8-61
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发表时间：
2008-04-15
期刊：
影响因子：
4.2
通讯作者：
Azad AF
中科院分区：
文献类型：
--
作者：
Dreher-Lesnick SM;Ceraul SM;Rahman MS;Azad AF
立克次体在多种真核宿主环境中生存的能力为研究病原体-宿主分子相互作用提供了良好的模型。鼠斑疹伤寒的病原立克次体是一种严格意义上的胞内革兰氏阴性α-变形菌，通过节肢动物媒介东方鼠蚤（Xenopsylla cheopis）传播给人类。因此，伤寒杆菌必须在哺乳动物和跳蚤宿主之间循环，这是两种截然不同的环境。我们假设温度在调节宿主特异性基因表达中起作用，使斑疹伤寒在哺乳动物和节肢动物宿主中存活。在这项研究中，我们使用Affymetrix微阵列在37°C到25°C的温度变化中筛选温度诱导基因，模拟体外两种不同的宿主温度。温度响应基因属于多种功能类别，其中包括转录，翻译，翻译后修饰/蛋白质周转/伴侣和细胞内运输和分泌。大量差异表达基因的特征仍然很差，要么没有已知的功能，要么不在COG数据库中。用实时定量RT-PCR验证微阵列结果。该微阵列筛选鉴定了在温度从37°C到25°C变化时差异表达的各种基因。对所鉴定基因的进一步鉴定可能为斑疹伤寒在哺乳动物和节肢动物宿主之间成功过渡的能力提供新的见解。
The ability of rickettsiae to survive in multiple eukaryotic host environments provides a good model for studying pathogen-host molecular interactions. Rickettsia typhi, the etiologic agent of murine typhus, is a strictly intracellular gram negative α-proteobacterium, which is transmitted to humans by its arthropod vector, the oriental rat flea, Xenopsylla cheopis. Thus, R. typhi must cycle between mammalian and flea hosts, two drastically different environments. We hypothesize that temperature plays a role in regulating host-specific gene expression, allowing R. typhi to survive in mammalian and arthropod hosts. In this study, we used Affymetrix microarrays to screen for temperature-induced genes upon a temperature shift from 37°C to 25°C, mimicking the two different host temperatures in vitro. Temperature-responsive genes belonged to multiple functional categories including among others, transcription, translation, posttranslational modification/protein turnover/chaperones and intracellular trafficking and secretion. A large number of differentially expressed genes are still poorly characterized, and either have no known function or are not in the COG database. The microarray results were validated with quantitative real time RT-PCR. This microarray screen identified various genes that were differentially expressed upon a shift in temperature from 37°C to 25°C. Further characterization of the identified genes may provide new insights into the ability of R. typhi to successfully transition between its mammalian and arthropod hosts.
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影响因子：
11.8
作者：
Azad, AF;Radulovic, S;Troyer, JM
通讯作者：
Troyer, JM
影响因子：
56.9
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OSTERMAN, JV
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