The Cloning and Functional Characterization of Peach CONSTANS and FLOWERING LOCUS T Homologous Genes PpCO and PpFT.
The Cloning and Functional Characterization of Peach CONSTANS and FLOWERING LOCUS T Homologous Genes PpCO and PpFT.
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桃CONSTANS和FLOWERING LOCUS T同源基因PpCO和PpFT的克隆及功能鉴定
DOI：
10.1371/journal.pone.0124108
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yu F
中科院分区：
文献类型：
--
作者：
Zhang X;An L;Nguyen TH;Liang H;Wang R;Liu X;Li T;Qi Y;Yu F
开花是植物生长发育的重要阶段。成功过渡到开花不仅确保了植物生命周期的完成，而且还是生产具有重要经济意义的种子和果实的基础。 CONSTANS (CO) 和 FLOWERING LOCUS T (FT) 是在拟南芥开花时间控制中发挥关键作用的两个基因。通过基于同源性的克隆和cDNA末端快速扩增（RACE），我们获得了桃（Prunus persica (L.) Batsch）的桃桃CO（PpCO）和桃桃FT（PpFT）的全长cDNA序列，并研究了它们在开花时间调控中的功能。 PpCO 和 PpFT 在 DNA、mRNA 和蛋白质水平上与拟南芥 CO 和 FT 表现出高度同源性。我们发现 PpCO 和 PpFT 是核定位的，并且都在酵母细胞中表现出转录激活活性，这与它们作为转录激活剂的潜在作用一致。此外，我们还发现PpCO的过表达可以恢复拟南芥co-2突变体的晚花表型，而拟南芥ft-1突变体的晚花缺陷可以通过PpFT的过表达来挽救，这表明桃和拟南芥中CO和FT基因的功能保守性。我们的结果表明，PpCO 和 PpFT 是桃中 CO 和 FT 的同源基因，它们可能在调节植物开花时间方面发挥作用。
Flowering is an essential stage of plant growth and development. The successful transition to flowering not only ensures the completion of plant life cycles, it also serves as the basis for the production of economically important seeds and fruits. CONSTANS (CO) and FLOWERING LOCUS T (FT) are two genes playing critical roles in flowering time control in Arabidopsis. Through homology-based cloning and rapid-amplifications of cDNA ends (RACE), we obtained full-lengths cDNA sequences of Prunus persica CO (PpCO) and Prunus persica FT (PpFT) from peach (Prunus persica (L.) Batsch) and investigated their functions in flowering time regulation. PpCO and PpFT showed high homologies to Arabidopsis CO and FT at DNA, mRNA and protein levels. We showed that PpCO and PpFT were nucleus-localized and both showed transcriptional activation activities in yeast cells, consistent with their potential roles as transcription activators. Moreover, we established that the over-expression of PpCO could restore the late flowering phenotype of the Arabidopsis co-2 mutant, and the late flowering defect of the Arabidopsis ft-1 mutant can be rescued by the over-expression of PpFT, suggesting functional conservations of CO and FT genes in peach and Arabidopsis. Our results suggest that PpCO and PpFT are homologous genes of CO and FT in peach and they may function in regulating plant flowering time.
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