Scaffoldless tissue-engineered nerve conduit promotes peripheral nerve regeneration and functional recovery after tibial nerve injury in rats.
Scaffoldless tissue-engineered nerve conduit promotes peripheral nerve regeneration and functional recovery after tibial nerve injury in rats.
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DOI：
10.4103/1673-5374.215265
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发表时间：
2017-09
影响因子：
6.1
通讯作者：
Larkin LM
中科院分区：
文献类型：
--
作者：
Adams AM;VanDusen KW;Kostrominova TY;Mertens JP;Larkin LM
周围神经组织的损伤可能会导致神经及其所支配的目标肌肉功能丧失。本研究比较了工程神经导管 (ENC)、工程成纤维细胞导管 (EFC) 和自体移植物在 10 毫米胫神经间隙中的修复能力。 ENC 是利用大鼠胚胎第 15 天 (E15) 的原代成纤维细胞和神经细胞制造的。 EFC 仅利用原代成纤维细胞制造。 12 周恢复后，通过测量腓肠肌内侧肌的收缩特性、腓肠肌外侧的远端运动神经传导速度以及肌肉和神经的组织学来评估神经修复。自体移植物、ENC和EFC分别重建了腓肠肌外侧原生远端运动神经传导速度的96%、87%和84%，医用腓肠肌原生比力的100%、44%和44%，以及原生腓肠肌内侧质量的63%、61%和67%。修复神经的组织学显示，自体移植物中存在较大的轴突，ENC 修复中存在较大但较少的轴突，EFC 修复中存在许多较小的轴突。肌肉组织学显示自体移植、ENC 和 EFC 修复之间的肌纤维横截面积相似。总之，ENC和EFC在10毫米胫神经间隙修复中均促进神经再生，这表明E15大鼠神经细胞可能不是神经再生所必需的，仅EFC就足以修复周围神经损伤。
Damage to peripheral nerve tissue may cause loss of function in both the nerve and the targeted muscles it innervates. This study compared the repair capability of engineered nerve conduit (ENC), engineered fibroblast conduit (EFC), and autograft in a 10-mm tibial nerve gap. ENCs were fabricated utilizing primary fibroblasts and the nerve cells of rats on embryonic day 15 (E15). EFCs were fabricated utilizing primary fibroblasts only. Following a 12-week recovery, nerve repair was assessed by measuring contractile properties in the medial gastrocnemius muscle, distal motor nerve conduction velocity in the lateral gastrocnemius, and histology of muscle and nerve. The autografts, ENCs and EFCs reestablished 96%, 87% and 84% of native distal motor nerve conduction velocity in the lateral gastrocnemius, 100%, 44% and 44% of native specific force of medical gastrocnemius, and 63%, 61% and 67% of native medial gastrocnemius mass, respectively. Histology of the repaired nerve revealed large axons in the autograft, larger but fewer axons in the ENC repair, and many smaller axons in the EFC repair. Muscle histology revealed similar muscle fiber cross-sectional areas among autograft, ENC and EFC repairs. In conclusion, both ENCs and EFCs promoted nerve regeneration in a 10-mm tibial nerve gap repair, suggesting that the E15 rat nerve cells may not be necessary for nerve regeneration, and EFC alone can suffice for peripheral nerve injury repair.
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影响因子：
4.1
作者：
Kemp, Stephen W. P.;Syed, M. A. Shahbaz;Midha, Rajiv
通讯作者：
Midha, Rajiv
影响因子：
--
作者：
Larkin, Lisa M.;Calve, Sarah;Arruda, Ellen M.
通讯作者：
Arruda, Ellen M.
影响因子：
2.9
作者：
Matsumoto, K;Ohnishi, K;Shimizu, Y
通讯作者：
Shimizu, Y
影响因子：
5.3
作者：
Lee, AC;Yu, VM;Sakiyama-Elbert, SE
通讯作者：
Sakiyama-Elbert, SE
影响因子：
2.9
作者：
Murakami, T;Fujimoto, Y;Ochi, M
通讯作者：
Ochi, M