Disposable inkjet-printed electrochemical platform for detection of clinically relevant HER-2 breast cancer biomarker.
Disposable inkjet-printed electrochemical platform for detection of clinically relevant HER-2 breast cancer biomarker.
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DOI：
10.1016/j.bios.2018.01.003
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发表时间：
2018-05-01
影响因子：
12.6
通讯作者：
Krause CE
中科院分区：
文献类型：
--
作者：
Carvajal S;Fera SN;Jones AL;Baldo TA;Mosa IM;Rusling JF;Krause CE
快速制造的一次性传感器平台为即时检测提供了巨大的希望。在这里，我们提出了一种廉价（< 0.25美元）的全喷墨印刷电化学传感器，集成了计数器，参考和工作电极，易于扩展用于商业制造。该电化学传感器平台具有喷墨印刷金工作8电极阵列（WEA）和对电极（CE），以及喷墨印刷银电极，该银电极用漂白剂氯化以产生Ag/AgCl准参比电极（RE）。作为概念验证，电化学传感器已成功应用于临床相关乳腺癌生物标志物人表皮生长因子受体2 （HER-2）的检测。将捕获的抗体与WEA的化学修饰表面结合，并放入微流体装置中。将目标蛋白、生物素化抗体和聚合的辣根过氧化物标签同时注射到装有WEA的微流体装置中，构建了一个完整的三明治免疫分析。超快速检测时间仅为15分钟，达到了12 pg mL−1的临床相关检测限。通过在人血清中进行的回收率测定，观察到良好的重现性和灵敏度，回收率为76-103%。这些易于制造和可扩展的电化学传感器平台可以很容易地适应多路检测，按照这种快速检测方案进行癌症诊断。
Rapidly fabricated, disposable sensor platforms hold tremendous promise for point-of-care detection. Here, we present an inexpensive (< $0.25) fully inkjet printed electrochemical sensor with integrated counter, reference, and working electrodes that is easily scalable for commercial fabrication. The electrochemical sensor platform featured an inkjet printed gold working 8-electrode array (WEA) and counter electrode (CE), along with an inkjet –printed silver electrode that was chlorinated with bleach to produce a Ag/AgCl quasi-reference electrode (RE). As proof of concept, the electrochemical sensor was successfully applied for detection of clinically relevant breast cancer biomarker Human Epidermal Growth Factor Receptor 2 (HER-2). Capture antibodies were bound to a chemically modified surface on the WEA and placed into a microfluidic device. A full sandwich immunoassay was constructed following a simultaneous injection of target protein, biotinylated antibody, and polymerized horseradish peroxide labels into the microfluidic device housing the WEA. With an ultra fast assay time, of only 15 mins a clinically relevant limit of detection of 12 pg mL−1 was achieved. Excellent reproducibility and sensitivity were observed through recovery assays preformed in human serum with recoveries ranging from 76–103%. These easily fabricated and scalable electrochemical sensor platforms can be readily adapted for multiplex detection following this rapid assay protocol for cancer diagnostics.
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影响因子：
19
作者：
Khan, Yasser;Pavinatto, Felippe J.;Arias, Ana C.
通讯作者：
Arias, Ana C.
影响因子：
19
作者：
Siegel, Adam C.;Phillips, Scott T.;Whitesides, George M.
通讯作者：
Whitesides, George M.
DOI：
10.1039/c0an00204f
发表时间：
2010-10
期刊：
The Analyst
影响因子：
--
作者：
Rusling JF;Kumar CV;Gutkind JS;Patel V
通讯作者：
Patel V
影响因子：
3
作者：
Munge BS;Stracensky T;Gamez K;DiBiase D;Rusling JF
通讯作者：
Rusling JF
影响因子：
9.3
作者：
Sturgeon, Catharine M.;Duffy, Michael J.;Diamandis, Eleftherios P.
通讯作者：
Diamandis, Eleftherios P.