Quantification of non-specific binding of magnetic micro- and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection.
Quantification of non-specific binding of magnetic micro- and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection.
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DOI：
10.1002/bit.22635
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发表时间：
2010-04-15
影响因子：
3.8
通讯作者：
Zborowski, M.
中科院分区：
文献类型：
--
作者：
Chalmers, J. J.;Xiong, Y.;Jin, X.;Shao, M.;Tong, X.;Farag, S.;Zborowski, M.
关键词：
磁性细胞分离技术的成熟对磁性细胞分离性能提出了越来越高的要求。虽然许多因素可能导致次优性能，但主要挑战之一可能是磁性纳米或微米颗粒与非靶向细胞的非特异性结合。根据分离的类型，这种非特异性结合可能对最终纯度、靶细胞的回收率或两者都有负面影响。在这项工作中，我们定量地证明了磁性纳米颗粒的非特异性结合可以赋予细胞磁化，使得这些细胞可以保留在分离柱中，从而对最终产品的纯度和所需细胞的回收产生负面影响。通过实验数据和理论论证，我们证明了赋予足以使非靶细胞保留在柱中的磁化所需的MACS磁性颗粒的数量为500至1，000个纳米颗粒。这个非特异性结合的粒子数是用细胞跟踪测速仪CTV实验证明的，并且证明了CTV仪器对磁性纳米粒子中所含Fe原子的灵敏度为1 × 10−15 g/mL Fe。
The maturation of magnetic cell separation technology places increasing demands on magnetic cell separation performance. While a number of factors can cause suboptimal performance, one of the major challenges can be non-specific binding of magnetic nano or micro particles to non-targeted cells. Depending on the type of separation, this non-specific binding can have a negative effect on the final purity, the recovery of the targeted cells, or both. In this work, we quantitatively demonstrate that non-specific binding of magnetic nanoparticles can impart a magnetization to cells such that these cells can be retained in a separation column and thus negatively impact the purity of the final product and the recovery of the desired cells. Through experimental data and theoretical arguments, we demonstrate that the number of MACS magnetic particles needed to impart a magnetization that is sufficient to causes non-targeted cells to be retained in the column to be on the order of 500 to 1,000 nanoparticles. This number of non-specifically bound particles was demonstrated experimentally with an instrument, cell tracking velocimeter, CTV, and it is demonstrated that the sensitivity of the CTV instrument for Fe atoms contained in magnetic nanoparticles on the order of 1 × 10−15 g/mL of Fe.
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影响因子：
3.8
作者：
Melnik, Kristie;Sun, Jianxin;Chalmers, Jeffrey J.
通讯作者：
Chalmers, Jeffrey J.
DOI：
10.1016/s0165-022x(00)00085-3
发表时间：
2000-07-10
影响因子：
--
作者：
Moore, LR;Zborowski, M;Chalmers, JJ
通讯作者：
Chalmers, JJ
影响因子：
4.2
作者：
Zhang, HD;Moore, LR;Chalmers, JJ
通讯作者：
Chalmers, JJ
影响因子：
4.2
作者：
Jin, Xiaoxia;Zhao, Yang;Chalmers, Jeffrey J.
通讯作者：
Chalmers, Jeffrey J.
影响因子：
2.7
作者：
Yamaura, M;Camilo, RL;Toma, HE
通讯作者：
Toma, HE