Proneural and abdominal Hox inputs synergize to promote sensory organ formation in the Drosophila abdomen.
Proneural and abdominal Hox inputs synergize to promote sensory organ formation in the Drosophila abdomen.
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DOI：
10.1016/j.ydbio.2010.09.014
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发表时间：
2010-12-15
影响因子：
2.7
通讯作者：
Gebelein B
中科院分区：
文献类型：
--
作者：
Gutzwiller LM;Witt LM;Gresser AL;Burns KA;Cook TA;Gebelein B
无调（ato）前神经基因指定了果蝇外胚层各体节内感觉器官前体（SOP）的刻板数量。令人惊讶的是，Ato在外胚层内的广泛表达仅导致SOP形成的适度增加，表明许多细胞不能成为SOP。在这里，我们表明，Ato的SOP促进活性可以通过三个因素大大增强：无意义（Sens）锌指蛋白，Abdominal-A（Abd-A）Hox因子和表皮生长因子（EGF）途径。首先，我们表明，无论是单独或与Sens的表达诱导的SOP在腹部的两倍，在胸部，这样做的代价是特定的细胞命运：幼虫卵母细胞。第二，我们证明，Ato刺激腹部SOP的形成，协同与Abd-A，以促进EGF配体（斯皮茨）分泌和二次SOP招聘。然而，我们还发现，Ato和Sens选择性地增强腹部SOP的发展，在Spitz独立的方式，这表明额外的遗传相互作用之间的proneural途径和Abd-A。总之，这些实验揭示了EGF信号传导，Abd-A，和Sens之间的遗传相互作用增强了Ato的SOP促进活性，以刺激果蝇腹部区域特异性神经发生。
The atonal (ato) proneural gene specifies a stereotypic number of sensory organ precursors (SOP) within each body segment of the Drosophila ectoderm. Surprisingly, the broad expression of Ato within the ectoderm results in only a modest increase in SOP formation, suggesting many cells are incompetent to become SOPs. Here, we show that the SOP promoting activity of Ato can be greatly enhanced by three factors: the Senseless (Sens) zinc finger protein, the Abdominal-A (Abd-A) Hox factor, and the epidermal growth factor (EGF) pathway. First, we show that expression of either Ato alone or with Sens induces twice as many SOPs in the abdomen as in the thorax, and do so at the expense of an abdomen-specific cell fate: the larval oenocytes. Second, we demonstrate that Ato stimulates abdominal SOP formation by synergizing with Abd-A to promote EGF ligand (Spitz) secretion and secondary SOP recruitment. However, we also found that Ato and Sens selectively enhance abdominal SOP development in a Spitz-independent manner, suggesting additional genetic interactions between this proneural pathway and Abd-A. Altogether, these experiments reveal that genetic interactions between EGF-signaling, Abd-A, and Sens enhance the SOP-promoting activity of Ato to stimulate region-specific neurogenesis in the Drosophila abdomen.
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影响因子：
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作者：
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通讯作者：
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