Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues.
Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues.
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DOI：
10.4142/jvs.2016.17.4.445
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发表时间：
2016-12-30
影响因子：
1.8
通讯作者：
Jeung EB
中科院分区：
文献类型：
--
作者：
Lee B;Kang HY;Lee DO;Ahn C;Jeung EB
紧密连接蛋白是一种跨膜蛋白，在紧密连接中起着重要的作用，形成物理屏障，调节细胞旁转运。为了了解马的疾病，这是有帮助的，以测量组织特异性表达的TJ在马。主要的马疾病，如绞痛和西尼罗河造成损害的TJ。本研究采用免疫印迹和免疫组织化学方法检测了claudin-1、claudin-2、claudin-4和claudin-5在8种组织中的表达水平和分布。Claudin-1主要在肺、十二指肠和子宫中鉴定，claudin-2在马组织中均匀观察到，claudin-4在肝、肾和子宫中大量检测到，claudin-5在肺、十二指肠、卵巢和子宫中强烈表达，如通过蛋白质印迹法测定的。用免疫组织化学方法观察马claudins的分布。这些发现提供了关于马claudins的表达模式和定位的知识，以及有价值的信息，以了解紧密连接相关疾病根据组织特异性和claudins在马中的功能。
Claudins, which are known as transmembrane proteins play an essential role in tight junctions (TJs) to form physical barriers and regulate paracellular transportation. To understand equine diseases, it is helpful to measure the tissue-specific expression of TJs in horses. Major equine diseases such as colic and West Nile cause damage to TJs. In this study, the expression level and distribution of claudin-1, -2, -4, and -5 in eight tissues were assessed by Western blotting and immunohistochemistry methods. Claudin-1 was primarily identified in the lung, duodenum, and uterus, claudin-2 was evenly observed in equine tissues, claudin-4 was abundantly detected in the liver, kidney and uterus, and claudin-5 was strongly expressed in the lung, duodenum, ovary, and uterus, as determined by Western blotting method. The localization of equine claudins was observed by immunohistochemistry methods. These findings provide knowledge regarding the expression patterns and localization of equine claudins, as well as valuable information to understand tight junction-related diseases according to tissue specificity and function of claudins in horses.
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影响因子：
7.8
作者：
Furuse, Mikio;Hata, Masaki;Furuse, Kyoko;Yoshida, Yoko;Haratake, Akinori;Sugitani, Yoshinobu;Noda, Tetsuo;Kubo, Akiharu;Tsukita, Shoichiro
通讯作者：
Tsukita, Shoichiro
影响因子：
7.8
作者：
Nitta, T;Hata, M;Tsukita, S
通讯作者：
Tsukita, S
DOI：
10.1083/jcb.147.1.185
发表时间：
1999-10-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Morita K;Sasaki H;Furuse M;Tsukita S
通讯作者：
Tsukita S
紧密连接链内和之间异质claudin物种相互作用的方式。
影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Tsukita, S
通讯作者：
Tsukita, S
DOI：
10.1083/jcb.141.7.1539
发表时间：
1998-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Furuse M;Fujita K;Hiiragi T;Fujimoto K;Tsukita S
通讯作者：
Tsukita S