Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique.
Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique.
复制标题
硅/铜掺杂高能喷丸辅助微弧氧化技术对钛进行抗菌和成骨功能化
DOI：
10.3389/fbioe.2020.573464
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发表时间：
2020
影响因子：
5.7
通讯作者：
Wu G
中科院分区：
文献类型：
--
作者：
Shen X;Hu W;Ping L;Liu C;Yao L;Deng Z;Wu G
钛（Ti）种植体的抗菌和成骨功能化将大大扩展其在即刻种植治疗中的临床适应症，加速骨整合，并改善长期预后。近年来，高能喷丸（HESP）辅助微弧氧化（MAO）技术显著提高了钛基材料的生物活性和膜层稳定性。在这项研究中，我们进一步功能化钛抗菌和成骨性能的掺杂硅（Si）和/或铜（Cu）离子到HESP/MAO处理的涂层。物理化学表征表明，硅和铜的掺杂在HESP/MAO处理涂层（Si/Cu-MAO）没有显着改变其表面形貌，粗糙度，晶体结构，涂层厚度，结合强度，和润湿性。X射线光电子能谱（XPS）结果表明，Si/Cu-MAO涂层中的Si和Cu分别以硅酸根（SiO 32-）和二价铜（Cu 2+）的形式存在。硅和铜的总量分别为13.5和5.8 μg/cm ~ 2，在14天内分别释放了33.2%和31.3%。与对照组（MAO）相比，硅掺杂样品（MAO-Si）显著提高了MC 3 T3-E1细胞的细胞活力、碱性磷酸酶（ALP）活性、矿化度和成骨基因（ALP、I型胶原和骨钙素）的表达。此外，铜的添加对金黄色葡萄球菌和变形链球菌都具有良好的杀菌性能（即使在细菌与MC 3 T3-E1细胞共培养的条件下）：对两种细菌的抑菌率均超过95%。总之，具有抗菌和成骨特性的新型生物活性Si/Cu-MAO涂层是骨科和牙科植入物的一种有前途的功能化方法，特别是在感染牙槽的即刻植入治疗中。
Antibacterial and osteogenic functionalization of titanium (Ti) implants will greatly expand their clinical indications in immediate implant therapy, accelerate osteointegration, and enhance long-term prognosis. We had recently shown that the high-energy shot peening (HESP)-assisted micro-arc oxidation (MAO) significantly improved the bioactivity and coating stability of Ti-based substrates. In this study, we further functionalized Ti with antibacterial and osteogenic properties by doping silicon (Si) and/or copper (Cu) ions into HESP/MAO-treated coatings. Physicochemical characterization displayed that the doping of Si and Cu in HESP/MAO-treated coatings (Si/Cu-MAO) did not significantly change their surface topography, roughness, crystal structure, coating thickness, bonding strength, and wettability. The results of X-ray photoelectron spectroscopy (XPS) showed that Si and Cu in the Si/Cu-MAO coating was in the form of silicate radical (SiO32–) and bivalent copper (Cu2+), respectively. The total amounts of Si and Cu were about 13.5 and 5.8 μg/cm2, which released about 33.2 and 31.3% within 14 day, respectively. Compared with the control group (MAO), Si doping samples (MAO-Si) significantly increased the cell viability, alkaline phosphatase (ALP) activity, mineralization and osteogenic genes (ALP, collagen I and osteocalcin) expression of MC3T3-E1 cells. Furthermore, the addition of Cu presented good bactericidal property against both Staphylococcus aureus and Streptococcus mutans (even under the co-culture condition of bacteria and MC3T3-E1 cells): the bacteriostatic rate of both bacteria was over 95%. In conclusion, the novel bioactive Si/Cu-MAO coating with antibacterial and osteogenic properties is a promising functionalization method for orthopedic and dental implants, especially in the immediate implant treatment with an infected socket.
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影响因子：
14
作者：
Abaricia, Jefferson O.;Shah, Arth H.;Olivares-Navarrete, Rene
通讯作者：
Olivares-Navarrete, Rene
影响因子：
3.9
作者：
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通讯作者：
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影响因子：
4.3
作者：
Kim, Beom-Su;Yang, Sun-Sik;Lee, Jun
通讯作者：
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影响因子：
9.5
作者：
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通讯作者：
Yang, Mengsu
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影响因子：
8
作者：
Shen, Xinkun;Al-Baadani, Mohammed A.;Liu, Jinsong
通讯作者：
Liu, Jinsong