Ventrally emigrating neural tube cells contribute to the formation of Meckel's and quadrate cartilage
Ventrally emigrating neural tube cells contribute to the formation of Meckel's and quadrate cartilage
复制标题
腹侧迁移神经管细胞有助于梅克尔软骨和方软骨的形成
作者：
G. S. Sohal;M. Ali;A.A. Ali;D. Dai
一群起源于后脑神经管腹侧部分的多电位神经上皮细胞最近被证明迁移到三叉神经的附着部位。这些向腹侧迁移的神经管细胞分布在第一咽（鳃）弓的间质中。因为梅克尔软骨和方形软骨是从这种间质发育而来的，我们试图确定这些腹侧迁移的神经管细胞是否促进了它们的发育。腹侧神经管细胞用含有LacZ基因的复制缺陷逆转录病毒载体进行标记。该方法永久标记神经管细胞后代；因此，可以在开发过程中跟踪它们。在胚胎第2天（第14期）神经嵴迁移完成后，将病毒浓缩液微注射到鸡胚吻侧后脑腔内。在对照胚胎中，病毒被放置在神经管的顶部。胚胎在第3,4和7天被杀死，并处理用于检测LacZ阳性细胞。到第7天，可以很容易地识别梅克尔软骨和方形软骨。两个软骨均可见LacZ阳性细胞。它们位于软骨膜和软骨中。神经嵴细胞标记物HNK‐1免疫染色显示LacZ‐阳性细胞为HNK‐1阴性。HNK‐1阳性神经嵴来源的细胞位于软骨中，而不在软骨膜中。这些结果表明，梅克尔软骨和方形软骨中的软骨细胞分化为两种细胞来源；向腹侧迁移的神经管细胞和神经嵴。软骨从腹侧神经管细胞分化的发育意义以及软骨细胞在形态发生中的异质起源仍有待确定。开发动态，1999;16:37 - 44。©1999 Wiley‐Liss, Inc。
A population of multipotential neuroepithelial cells originating in the ventral portion of the hindbrain neural tube has been shown recently to emigrate at the site of attachment of the trigeminal nerve. These ventrally emigrating neural tube cells populate the mesenchyme of the first pharyngeal (branchial) arch. Because the Meckel's and the quadrate cartilage develop from this mesenchyme, we sought to determine whether these ventrally emigrating neural tube cells contributed to their development. The ventral neural tube cells were tagged with a replication‐deficient retroviral vector containing the LacZ gene. This method permanently labels the descendents of the neural tube cells; thus, they can be subsequently tracked during development. The viral concentrate was microinjected into the lumen of the rostral hindbrain of chick embryos, after the emigration of neural crest is finished, on embryonic day 2 (stage 14). In control embryos, the virus was placed on top of the neural tube. Embryos were killed on days 3, 4, and 7 and processed for the detection of LacZ‐positive cells. By day 7, the Meckel's and the quadrate cartilage can be easily recognized. LacZ‐positive cells were seen in both cartilages. They were located in perichondrium and in the cartilage. Immunostaining with the neural crest cell marker HNK‐1 indicated that the LacZ‐positive cells were HNK‐1 negative. The HNK‐1‐positive neural crest–derived cells were located in the cartilage but not in the perichondrium. These results indicate that the chondrocytes in the Meckel's and the quadrate cartilage differentiate from two sources of cells; the ventrally emigrating neural tube cells and the neural crest. The developmental significance of differentiation of cartilage from the ventral neural tube cells and of the heterogeneous origin of chondrocytes in morphogenesis remains to be established. Dev Dyn 1999;216:37–44. © 1999 Wiley‐Liss, Inc.
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影响因子：
3.1
作者：
Sohal,GS;Ali,AA;Ali,MM
通讯作者：
Ali,MM
神经皱襞消融后颅神经管的调节反应：神经嵴再生的时空性质和 Slug 的上调。
DOI：
10.1242/dev.121.12.4103
发表时间：
1995
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Sechrist,J;Nieto,MA;Zamanian,RT;Bronner-Fraser,M
通讯作者：
Bronner-Fraser,M
影响因子：
4.6
作者：
M. Selleck;M. Bronner‐Fraser
通讯作者：
M. Selleck;M. Bronner‐Fraser
DOI：
10.1006/bbrc.1998.0109
发表时间：
1999
期刊：
Biochemical and biophysical research communications.
影响因子：
--
作者：
Sohal,GS;Ali,MM;Ali,AA;Dai,D
通讯作者：
Dai,D
DOI：
10.1242/dev.118.4.1049
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Scherson,T;Serbedzija,G;Fraser,S;Bronner-Fraser,M
通讯作者：
Bronner-Fraser,M