European bat lyssavirus in Scottish bats.
European bat lyssavirus in Scottish bats.
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DOI：
10.3201/eid1104.040920
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发表时间：
2005-04
影响因子：
11.8
通讯作者：
Fooks AR
中科院分区：
文献类型：
--
作者：
Brookes SM;Aegerter JN;Smith GC;Healy DM;Jolliffe TA;Swift SM;Mackie IJ;Pritchard JS;Racey PA;Moore NP;Fooks AR
蝙蝠可能很少栖息在人类住宅中，因此人类接触的风险很低。我们报告的第一个血清阳性率研究的发生特异性抗体欧洲蝙蝠狂犬病病毒2型（EBLV-2）在Dauzion的蝙蝠。蝙蝠是从苏格兰东部和南部的19个地点捕获的。对198只Dauzon蝙蝠、20只Natterer蝙蝠和6只伏翼蝙蝠的样本进行了EBLV-2检测。对血样（N = 94）进行改良荧光抗体病毒中和试验，以测定抗体滴度。从0.05%到3.8%（95%置信区间）的Daurehon的蝙蝠是血清阳性。在检测的其他2个种属中未检出EBLV-2抗体。获得口腔拭子（N = 218），提取RNA进行逆转录-聚合酶链反应（RT-PCR）。RT-PCR包括用于核糖体RNA的泛狂犬病毒引物（N基因）和内部PCR对照引物。EBLV-2 RNA在任何测试的唾液样本中均未检测到，在病毒分离测试中也未检测到活病毒。
Daubenton bats may roost infrequently in human dwellings, so risk for human contact is low. We report the first seroprevalence study of the occurrence of specific antibodies to European bat lyssavirus type 2 (EBLV-2) in Daubenton's bats. Bats were captured from 19 sites across eastern and southern Scotland. Samples from 198 Daubenton's bats, 20 Natterer's bats, and 6 Pipistrelle's bats were tested for EBLV-2. Blood samples (N = 94) were subjected to a modified fluorescent antibody virus neutralization test to determine antibody titer. From 0.05% to 3.8% (95% confidence interval) of Daubenton's bats were seropositive. Antibodies to EBLV-2 were not detected in the 2 other species tested. Mouth swabs (N = 218) were obtained, and RNA was extracted for a reverse transcription–polymerase chain reaction (RT-PCR). The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA. EBLV-2 RNA was not detected in any of the saliva samples tested, and live virus was not detected in virus isolation tests.
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DOI：
10.1111/j.1439-0450.2004.00730.x
发表时间：
2004-03-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Vos, A;Müller, T;Fooks, AR
通讯作者：
Fooks, AR
影响因子：
2.2
作者：
Johnson, N;Selden, D;Fooks, AR
通讯作者：
Fooks, AR
影响因子：
6.4
作者：
SIMS, RA;SULKIN, SE;ALLEN, R
通讯作者：
ALLEN, R
影响因子：
1.3
作者：
PEREZJORDA, JL;IBANEZ, C;TELLEZ, A
通讯作者：
TELLEZ, A
影响因子：
3.1
作者：
Smith, J;McElhinney, LM;Lowings, JP
通讯作者：
Lowings, JP