A new function of a previously isolated compound that stimulates activation and differentiation of myogenic precursor cells leading to efficient myofiber regeneration and muscle repair.
A new function of a previously isolated compound that stimulates activation and differentiation of myogenic precursor cells leading to efficient myofiber regeneration and muscle repair.
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先前分离的化合物的新功能，可刺激肌原性前体细胞的激活和分化，从而实现有效的肌纤维再生和肌肉修复。
DOI：
10.1016/j.biocel.2005.12.004
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
严重损伤后的肌肉修复是缓慢和不完全的，这是由于包括该功能的肌肉的再生能力有限。本研究从Geum japonicum中分离得到一个结构类似于三萜类化合物的化合物，该化合物能直接诱导静止卫星细胞在体外活化、增殖和成熟为心肌细胞。在严重肌肉损伤的修复中进一步测试了该化合物对肌生成的潜在作用。发现该化合物可显著刺激受损肌肉的再生潜能，导致肌管和肌管束的再生，其时间依赖性地替代受损肌肉组织。这种化合物介导的修复受损肌肉的新肌纤维的主动再生可能是由于其对静止肌源性前体细胞的活化和增殖的直接作用以及它们成熟为再生肌管的增强，如在我们的原代肌源性前体细胞培养实验中所证明的。化合物处理的肌源性前体细胞中内源性磷酸化Akt1的上调表达也可能通过促进肌源性细胞存活能力而有助于肌纤维再生和肌肉修复的过程。
Muscle repair following severe injury is slow and incomplete due to the limited regenerative capacity of muscles comprising the function. In this study, one pure compound structurally corresponding to triterpenoid, which can directly induce the activation, proliferation and maturation of quiescent satellite cells into myocytes in vitro, was isolated from Geum japonicum. The potential effect of this compound on myogenesis was further tested in repair of severe muscle injury. It was found that this compound could significantly stimulate the regenerative potential of the damaged muscle resulting in regeneration of myotubes and myotube bundles time-dependently replacing the damaged muscle tissues. This compound-mediated active regeneration of new myofibers repairing damaged muscles was probably due to its direct action on activation and proliferation of quiescent myogenic precursor cells and enhancement of their maturation into regenerating myotubes, as was demonstrated in our primary myogenic precursor cells culture experiments. The up-regulated expression of endogenous phospho-Akt1 in compound-treated myogenic precursor cells may also contribute to the process of myofiber regeneration and muscle repair probably via promoting myogenic cell survival capacity.
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影响因子：
3.9
作者：
M. Li;K. Chan;D. Cai;P. Leung;C. Cheng;K. Lee;K. K. Lee-K.
通讯作者：
M. Li;K. Chan;D. Cai;P. Leung;C. Cheng;K. Lee;K. K. Lee-K.
影响因子：
7.8
作者：
Gulati, A K;Reddi, A H;Zalewski, A A
通讯作者：
Zalewski, A A
影响因子：
5.1
作者：
Dong, H;Chen, SX;Xu, HX
通讯作者：
Xu, HX
影响因子：
7
作者：
Machida, S;Booth, FW
通讯作者：
Booth, FW
影响因子：
2.7
作者：
RICHLER, C;YAFFE, D
通讯作者：
YAFFE, D