Increased formate-dependent H2 production from xylose in Enterobacter sp. CN1 transformed with a formate hydrogenlyase activator gene
Increased formate-dependent H2 production from xylose in Enterobacter sp. CN1 transformed with a formate hydrogenlyase activator gene
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肠杆菌属中木糖产生的甲酸盐依赖性 H2 增加。
DOI：
10.1063/1.4900548
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发表时间：
2014-10
影响因子：
2.5
通讯作者：
Hu Zhong
中科院分区：
文献类型：
--
作者：
Nian Dinglan;Zhang Mingming;Long Minnan;Hu Zhong
寻找高效产氢菌株和优化发酵条件是促进生物制氢工业化应用的两个主要途径。在本研究中，我们进行了实验，以检查的因素，影响H2产率在肠杆菌CN 1。结果表明，甲酸盐可以单独产氢，产率与甲酸盐浓度呈正相关。此外，在甲酸盐存在下，木糖产H2的产量显著增加，表明甲酸盐不仅是良好的碳源，而且促进菌株CN 1中木糖产H2。pH对甲酸发酵产H2的影响不大，但无论培养基中是否存在甲酸，木糖发酵产H2的pH值均高于木糖发酵产H2的pH值。此外，H2生产丙酮酸盐完全抑制时，次磷酸钠（HPP）的存在，但甲酸依赖的H2生产大多不受影响。此外，从葡萄糖或木糖产生H2显着减少，但在HPP存在下仍然可检测到，表明甲酸氢解酶是肠杆菌属CN 1中负责从木糖产生H2的主要途径。为了证实上述假设，甲酸氢裂解酶激活剂（fhlA）基因被克隆并在菌株CN 1中过表达。与野生型相比，重组CN1菌株在木糖存在下每克木糖和每克细胞的H2产量分别提高了10.5%和18.8%。
Finding bacterial strains for effective hydrogen production and optimization of the fermentative conditions are two major approaches to promoting commercial application of bio-hydrogen. In the present study, we carried out experiments to examine factors that affected H2 yield in Enterobacter sp. CN1. The result showed that H2 could be produced from formate alone and the yields were positively correlated with formate concentrations. Moreover, the yield of H2 from xylose was significantly increased in the presence of formate, revealing that formate is not only a good carbon source but also promotes H2 production from xylose in strain CN1. The production of H2 from formate was not affected by pH; however, H2 production from xylose at pH 7 was higher than that at pH 6, no matter if formate is presence or absence in the medium. Further, H2 production from pyruvate was inhibited completely when sodium hypophosphate (HPP) was present, but formate-dependent H2 production was mostly not affected. In addition, H2 production from glucose or xylose was dramatically reduced but sill detectable in the presence of HPP, suggesting that formate hydrogenlyase is the main pathway responsible for H2 production from xylose in Enterobacter sp. CN1. To confirm the above hypothesis, the formate hydrogenlyase activator (fhlA) gene was cloned and over-expressed in strain CN1. Compared with the wild-type, recombinant CN1 strain increased H2 production per gram of xylose and per gram of cells by 10.5% and 18.8%, respectively, in the presence of xylose.
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影响因子：
3.2
作者：
Roe, AJ;McLaggan, D;Booth, IR
通讯作者：
Booth, IR
影响因子：
7.2
作者：
Tanisho, S;Kuromoto, M;Kadokura, N
通讯作者：
Kadokura, N
影响因子：
11.4
作者：
Logan, BE;Oh, SE;Van Ginkel, S
通讯作者：
Van Ginkel, S
影响因子：
5
作者：
Kien Trung Tran;Maeda, Toshinari;Wood, Thomas K.
通讯作者：
Wood, Thomas K.
影响因子：
46.9
作者：
Benemann, J
通讯作者：
Benemann, J