Double-stranded RNA binding protein, Staufen, is required for the initiation of RNAi in coleopteran insects.
Double-stranded RNA binding protein, Staufen, is required for the initiation of RNAi in coleopteran insects.
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DOI：
10.1073/pnas.1809381115
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发表时间：
2018-08-14
影响因子：
11.1
通讯作者：
Palli SR
中科院分区：
文献类型：
--
作者：
Yoon JS;Mogilicherla K;Gurusamy D;Chen X;Chereddy SCRR;Palli SR
在线虫中发现的RNA干扰(RNAi)为基础科学和应用科学的重大进步做出了贡献。目前正在开发基于RNAi的方法来控制害虫和病媒。RNAi在鞘翅目昆虫中是高效和系统的，但在其他昆虫中不是。RNAi在重要经济昆虫中的效率较低，以及对抗药性发展的担忧阻碍了这项技术的广泛使用。为了解决这些问题，利用对RNAi敏感的科罗拉多州马铃薯甲虫Leptinotarsa decemlineata和从该昆虫衍生的细胞系来鉴定dsRNA结合蛋白StaufenC是RNAi及其抗性的主要贡献者。有趣的是，StaufenC同源物只存在于鞘翅目昆虫中，对于这些昆虫的有效RNAi反应及其抗性是必不可少的。RNA干扰(RNAi)正被用于开发控制害虫和病媒的方法。RNAi在鞘翅目昆虫中是强大的和系统的，但在其他昆虫中是非常不同的。鞘翅目昆虫中有效的RNAi的决定因素及其潜在的抗性机制尚不清楚。RNAi筛选发现一个双链RNA结合蛋白(StaufenC)是RNAi中的主要角色。只在鞘翅目昆虫中发现了StaufenC同源物。在两种鞘翅目昆虫上的实验表明，StaufenC对RNAi，特别是双链RNA(DsRNA)对小干扰RNA的加工是必需的。通过将Lepd-SL1细胞与凋亡抑制因子1 dsRNA作用于Lepd-SL1细胞多代，筛选出RNAi抗性细胞。与敏感细胞相比，耐药细胞的StaufenC表达水平较低。这些研究表明，鞘翅目昆虫特异的StaufenC是RNAi所必需的，是RNAi抗性的潜在靶标。本文中包含的数据将有助于改进非鞘翅目昆虫的RNAi，并管理鞘翅目昆虫的RNAi抗性。
The RNA interference (RNAi) discovered in nematodes has contributed to major advances in basic and applied sciences. RNAi-based methods are being developed for controlling pests and disease vectors. RNAi is highly efficient and systemic in coleopteran insects, but not in other insects. The lower efficiency of RNAi in economically important insects and concerns about resistance development are hindering the widespread use of this technology. To address these problems, a RNAi-sensitive Colorado potato beetle, Leptinotarsa decemlineata, and a cell line derived from this insect were used to identify a dsRNA-binding protein, StaufenC, as a major contributor to RNAi and its resistance. Interestingly, StaufenC homologs are present in only coleopteran insects and are essential for efficient RNAi response and its resistance in these insects. RNA interference (RNAi) is being used to develop methods to control pests and disease vectors. RNAi is robust and systemic in coleopteran insects but is quite variable in other insects. The determinants of efficient RNAi in coleopterans, as well as its potential mechanisms of resistance, are not known. RNAi screen identified a double-stranded RNA binding protein (StaufenC) as a major player in RNAi. StaufenC homologs have been identified in only coleopteran insects. Experiments in two coleopteran insects, Leptinotarsa decemlineata and Tribolium castaneum, showed the requirement of StaufenC for RNAi, especially for processing of double-stranded RNA (dsRNA) to small interfering RNA. RNAi-resistant cells were selected by exposing L. decemlineata, Lepd-SL1 cells to the inhibitor of apoptosis 1 dsRNA for multiple generations. The resistant cells showed lower levels of StaufenC expression compared with its expression in susceptible cells. These studies showed that coleopteran-specific StaufenC is required for RNAi and is a potential target for RNAi resistance. The data included in this article will help improve RNAi in noncoleopteran insects and manage RNAi resistance in coleopteran insects.
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影响因子：
3.7
作者：
Khajuria C;Ivashuta S;Wiggins E;Flagel L;Moar W;Pleau M;Miller K;Zhang Y;Ramaseshadri P;Jiang C;Hodge T;Jensen P;Chen M;Gowda A;McNulty B;Vazquez C;Bolognesi R;Haas J;Head G;Clark T
通讯作者：
Clark T
影响因子：
46.9
作者：
Kennerdell, JR;Carthew, RW
通讯作者：
Carthew, RW
影响因子：
64.5
作者：
STJOHNSTON, D;BEUCHLE, D;NUSSLEINVOLHARD, C
通讯作者：
NUSSLEINVOLHARD, C
影响因子：
64.5
作者：
Kim, YK;Furic, L;Maquat, LE
通讯作者：
Maquat, LE
DOI：
10.1079/pavsnnr20127004
发表时间：
2012-01-01
期刊：
CAB Reviews
影响因子：
--
作者：
Palli, S. R.
通讯作者：
Palli, S. R.