Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte.
Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte.
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DOI：
10.1371/journal.pone.0197059
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Clark T
中科院分区：
文献类型：
--
作者：
Khajuria C;Ivashuta S;Wiggins E;Flagel L;Moar W;Pleau M;Miller K;Zhang Y;Ramaseshadri P;Jiang C;Hodge T;Jensen P;Chen M;Gowda A;McNulty B;Vazquez C;Bolognesi R;Haas J;Head G;Clark T
利用dsRNA通过RNA干扰（RNAi）途径控制害虫正在被全球研究人员探索。然而，随着每一类新的昆虫防治化合物的出现，昆虫抗性的演变需要考虑，了解抗性机制对于设计持久的技术和有效的抗性管理策略至关重要。为了深入了解昆虫对dsRNA的抗性，采用田间筛选和随后的实验室选择方法，建立了玉米主要害虫DvSnf7抗性玉米根虫（Diabrotica virgifera virgifera）群体。对摄入的DvSnf7 dsRNA产生抗性的WCR损害了腔内摄取，并且抗性不是DvSnf7 dsRNA特异性的，正如对所有其他dsRNA的交叉抗性所表明的那样。对苏云金芽孢杆菌Cry3Bb1蛋白无抗性。DvSnf7 dsRNA耐药是隐性遗传，位于单位点和常染色体上。这些发现将为dsRNA在害虫防治中的应用提供新的见解。
The use of dsRNA to control insect pests via the RNA interference (RNAi) pathway is being explored by researchers globally. However, with every new class of insect control compounds, the evolution of insect resistance needs to be considered, and understanding resistance mechanisms is essential in designing durable technologies and effective resistance management strategies. To gain insight into insect resistance to dsRNA, a field screen with subsequent laboratory selection was used to establish a population of DvSnf7 dsRNA-resistant western corn rootworm, Diabrotica virgifera virgifera, a major maize insect pest. WCR resistant to ingested DvSnf7 dsRNA had impaired luminal uptake and resistance was not DvSnf7 dsRNA-specific, as indicated by cross resistance to all other dsRNAs tested. No resistance to the Bacillus thuringiensis Cry3Bb1 protein was observed. DvSnf7 dsRNA resistance was inherited recessively, located on a single locus, and autosomal. Together these findings will provide insights for dsRNA deployment for insect pest control.
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DOI：
10.1261/rna.048116.114
发表时间：
2015-05
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Ivashuta S;Zhang Y;Wiggins BE;Ramaseshadri P;Segers GC;Johnson S;Meyer SE;Kerstetter RA;McNulty BC;Bolognesi R;Heck GR
通讯作者：
Heck GR
影响因子：
3.7
作者：
Gassmann AJ;Petzold-Maxwell JL;Keweshan RS;Dunbar MW
通讯作者：
Dunbar MW
影响因子：
2.2
作者：
Gassmann, Aaron J.;Shrestha, Ram B.;St Clair, Coy R.
通讯作者：
St Clair, Coy R.
影响因子：
4.6
作者：
Jakka, Siva R. K.;Shrestha, Ram B.;Gassmann, Aaron J.
通讯作者：
Gassmann, Aaron J.
影响因子：
3.8
作者：
Fishilevich, Elane;Velez, Ana M.;Siegfried, Blair D.
通讯作者：
Siegfried, Blair D.