Unraveling flp-11/flp-32 dichotomy in nematodes.
Unraveling flp-11/flp-32 dichotomy in nematodes.
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DOI：
10.1016/j.ijpara.2016.05.010
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发表时间：
2016-10
影响因子：
4
通讯作者：
Mousley, Angela
中科院分区：
文献类型：
--
作者：
Atkinson, Louise E.;Miskelly, Iain R.;Moffett, Christy L.;McCoy, Ciaran J.;Maule, Aaron G.;Marks, Nikki J.;Mousley, Angela
flp-11在秀丽隐杆线虫中的表达模式受到限制。flp-11的表达在多种线虫中是保守和受限制的。Flp-32表达更广泛，并且在空间上具有物种特异性。在仅表达FlP-11的物种中，FlP-11的表达与FlP-32的分布更相似。FLP-11肽抑制多种线虫物种的运动功能。FMRFamide样肽（FLP）信号系统是线虫神经肌肉功能的核心。与线虫FLP/FLP受体生物学相关的新药发现工作是通过积累基本生物学数据来推进的，这些数据可以揭示神经肽能系统内的微妙复杂性。本研究报告了FMRFamide样肽编码基因-11（flp-11）和FMRFamide样肽编码基因-32（flp-32）的特征，这两种不同的flp基因编码多种线虫物种中的类似肽AMRN（A/S）LVRFamide-这是线虫FLPergic系统中唯一已知的这种现象的例子。利用生物信息学、原位杂交、免疫细胞化学和行为分析，我们发现：（i）flp-11和flp-32是不同的flp基因，它们在多个线虫物种中单独或串联表达，它们编码高度相似的肽;（ii）flp-11似乎不是秀丽隐杆线虫中最广泛表达的flp;（iii）在同时表达flp-11和flp-32的物种中，flp-11在线虫进化枝和生活方式中显示出保守的、受限制的表达模式;（iv）在同时表达flp-11和flp-32的物种中，flp-32的表达比flp-11更广泛且更不保守;（v）在仅表达flp-11的物种中，flp-11表达谱与在表达两者的物种中观察到的flp-32谱更相似;和（vi）FLP-11肽抑制多种线虫物种中的运动功能。flp-11和flp-32肽重复的生物学意义和进化起源仍然不清楚，尽管试图确定一个共同的祖先，这可能会变得更加清晰的基因组数据的可用性提高。这项工作深入了解了线虫神经肽能系统的复杂性，并开始研究线虫如何补偿结构神经元的简单性。从寄生虫控制的角度来看，这项工作强调了基础生物学数据的重要性，并对C。线虫作为寄生虫神经生物学的模型。
flp-11 expression pattern is restricted in Caenorhabditis elegans. flp-11 expression is conserved and restricted across multiple nematodes. flp-32 expression is more widespread and spatially species-specific. flp-11 expression is more similar to the flp-32 profile in species expressing only flp-11. FLP-11 peptides inhibit motor function in multiple nematode species. FMRFamide-like peptide (FLP) signalling systems are core to nematode neuromuscular function. Novel drug discovery efforts associated with nematode FLP/FLP receptor biology are advanced through the accumulation of basic biological data that can reveal subtle complexities within the neuropeptidergic system. This study reports the characterisation of FMRFamide-like peptide encoding gene-11 (flp-11) and FMRFamide-like peptide encoding gene-32 (flp-32), two distinct flp genes which encode the analogous peptide, AMRN(A/S)LVRFamide, in multiple nematode species – the only known example of this phenomenon within the FLPergic system of nematodes. Using bioinformatics, in situ hybridisation, immunocytochemistry and behavioural assays we show that: (i) flp-11 and -32 are distinct flp genes expressed individually or in tandem across multiple nematode species, where they encode a highly similar peptide; (ii) flp-11 does not appear to be the most widely expressed flp in Caenorhabditis elegans; (iii) in species expressing both flp-11 and flp-32, flp-11 displays a conserved, restricted expression pattern across nematode clades and lifestyles; (iv) in species expressing both flp-11 and flp-32, flp-32 expression is more widespread and less conserved than flp-11; (v) in species expressing only flp-11, the flp-11 expression profile is more similar to the flp-32 profile observed in species expressing both; and (vi) FLP-11 peptides inhibit motor function in multiple nematode species. The biological significance and evolutionary origin of flp-11 and -32 peptide duplication remains unclear despite attempts to identify a common ancestor; this may become clearer as the availability of genomic data improves. This work provides insight into the complexity of the neuropeptidergic system in nematodes, and begins to examine how nematodes may compensate for structural neuronal simplicity. From a parasite control standpoint, this work underscores the importance of basic biological data, and has wider implications for the utility of C. elegans as a model for parasite neurobiology.
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影响因子：
30.8
作者：
Jex, Aaron R.;Nejsum, Peter;Schwarz, Erich M.;Hu, Li;Young, Neil D.;Hall, Ross S.;Korhonen, Pasi K.;Liao, Shengguang;Thamsborg, Stig;Xia, Jinquan;Xu, Pengwei;Wang, Shaowei;Scheerlinck, Jean-Pierre Y.;Hofmann, Andreas;Sternberg, Paul W.;Wang, Jun;Gasser, Robin B.
通讯作者：
Gasser, Robin B.
影响因子：
4.8
作者：
Kimber, Michael J.;McKinney, Susan;Maule, Aaron G.
通讯作者：
Maule, Aaron G.
影响因子：
3.4
作者：
Maule, Aaron G.;Mousley, Angela;Halton, David W.
通讯作者：
Halton, David W.
影响因子：
2.4
作者：
DAY, TA;MAULE, AG;PAX, RA
通讯作者：
PAX, RA
影响因子：
56.9
作者：
HORVITZ, HR;CHALFIE, M;EVANS, PD
通讯作者：
EVANS, PD