Serum levels of inflammatory and regulatory cytokines in patients with hemorrhagic fever with renal syndrome.
Serum levels of inflammatory and regulatory cytokines in patients with hemorrhagic fever with renal syndrome.
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DOI：
10.1186/1471-2334-11-142
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发表时间：
2011-05-23
影响因子：
3.7
通讯作者：
Avšič-Županc T
中科院分区：
文献类型：
--
作者：
Saksida A;Wraber B;Avšič-Županc T
汉坦病毒是两种人畜共患疾病的病原体：肾综合征出血热（HFRS）和汉坦病毒心肺综合征（HCPS）。对HFRS的发病机制知之甚少。然而，有研究表明，免疫机制，包括细胞因子，可能在HFRS发病机制中发挥重要作用。因此，我们研究的目的是调查斯洛文尼亚HFRS患者血清样本中的细胞因子谱，并探讨细胞因子水平与疾病严重程度之间可能的相关性。本研究包括来自52名诊断为DOBV感染的患者和61名诊断为PUUV感染的患者的急性期血清样本。根据疾病的严重程度将患者分为两组-重度或轻度。用ELISA法检测血清中IL-10、IL-12、INF-γ和TNF-α的水平。在几乎所有检测的血清样本中均发现IL-10、INF-γ和TNF-α水平升高。平均而言，在感染DOBV的患者中检测到的浓度高于PUUV。此外，在临床病程更严重的患者中发现了显著更高水平的IL-10（P = 0.001）和TNF-α（P = 0.003）。当仅考虑DOBV感染患者时，也观察到IL-10（P < 0.001）和TNF-α（P = 0.021）与疾病严重程度的相关性。在感染PUUV的患者中，根据疾病严重程度观察到细胞因子浓度无差异。HFRS患者血清IL-12水平在正常范围内，但PUUV感染者血清IL-12水平高于DOBV感染者。我们认为促炎细胞因子和调节细胞因子的产生不平衡可能是导致HFRS更严重病程的部分原因。
Hantaviruses are the causative agents of two zoonotic diseases: hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). The pathogenesis of HFRS is poorly understood. However, it has been suggested that immune mechanisms, including cytokines, might have an important role in HFRS pathogenesis. Thus, the aim of our study was to investigate cytokine profiles in serum samples of HFRS patients from Slovenia and explore a possible correlation between cytokine levels and disease severity. Acute-phase serum samples from 52 patients, diagnosed with DOBV infection, and 61 patients, diagnosed with PUUV infection, were included in this study. Patients were divided into two groups - severe or mild - based on disease severity. Levels of IL-10, IL-12, INF-γ and TNF-α were measured in the serum samples with commercial ELISA tests. Increased levels of IL-10, INF-γ, and TNF-α were found in almost all the serum samples tested. On average, higher concentrations were detected in patients infected with DOBV than PUUV. Furthermore, significantly higher levels of IL-10 (P = 0.001) and TNF-α (P = 0.003) were found in patients with a more severe clinical course of disease. The same association between IL-10 (P < 0.001) and TNF-α (P = 0.021), and the severity of the disease was observed also when only patients infected with DOBV were considered. No differences in cytokine concentrations according to disease severity were observed in patients infected with PUUV. Concentrations of serum IL-12 in HFRS patients were in the normal range, however, higher levels were detected in patients infected with PUUV than in patients infected with DOBV. We suggest that imbalance in production of proinflammatory and regulatory cytokines might be in part responsible for a more severe course of HFRS.
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影响因子：
5.4
作者：
Klingström, J;Plyusnin, A;Lundkvist, Å
通讯作者：
Lundkvist, Å
影响因子：
6.4
作者：
Mori, M;Rothman, AL;Ennis, FA
通讯作者：
Ennis, FA
影响因子：
6.4
作者：
Green, S;Vaughn, DW;Ennis, FA
通讯作者：
Ennis, FA
影响因子：
11.8
作者：
Avsic-Zupanc, T;Petrovec, M;Lundkvist, Å
通讯作者：
Lundkvist, Å
DOI：
10.4269/ajtmh.1999.60.85
发表时间：
1999-01-01
影响因子：
3.3
作者：
Marta, RF;Montero, VS;Molinas, FC
通讯作者：
Molinas, FC