Operons are a conserved feature of nematode genomes.
Operons are a conserved feature of nematode genomes.
复制标题
DOI：
10.1534/genetics.114.162875
复制
发表时间：
2014-08
期刊：
影响因子：
3.3
通讯作者：
Müller B
中科院分区：
文献类型：
--
作者：
Pettitt J;Philippe L;Sarkar D;Johnston C;Gothe HJ;Massie D;Connolly B;Müller B
将基因组织成操纵子，即共转录产生多顺反子前mRNAs的基因簇，是一种在包括多个动物门在内的广泛的真核生物群体中发现的特征。操纵子存在于Chromadorea纲，这是两个主要线虫纲之一，但它们在另一个纲Enoplea中的分布尚不清楚。我们已经调查了旋毛虫、旋毛虫和鞭毛虫的基因组，并在Enoplea的成员中鉴定了第一个可能的操纵子。与其他线虫的多顺反子RNA解析机制一致，旋毛虫和小鼠旋毛虫操纵子第一个基因下游产生的mRNAs被反式剪接到剪接的前导RNA，我们能够检测到来自这些操纵子的多顺反子RNA。重要的是，当表达为秀丽线虫嵌合操纵子的一部分时，这些潜在的包涵体操纵子之一的推测的顺反子间区域赋予多顺反子加工活性。我们发现，位于操纵子中的旋毛虫基因具有更高的操纵子线虫同源物的可能性。然而，操纵子的结构在共性和基因含量方面在两个分类群之间并不是紧密保守的，这与操纵子的进化模式是一致的。尽管如此，我们还是发现了在Enoplea和Chromadorea之间保守的假定操纵子。我们的数据表明，操纵子和“剪接先导”(SL)的反式剪接早于线虫门的辐射，这一推断得到了已知参与线虫SL反式剪接的蛋白质系统发育谱的支持。
The organization of genes into operons, clusters of genes that are co-transcribed to produce polycistronic pre-mRNAs, is a trait found in a wide range of eukaryotic groups, including multiple animal phyla. Operons are present in the class Chromadorea, one of the two main nematode classes, but their distribution in the other class, the Enoplea, is not known. We have surveyed the genomes of Trichinella spiralis, Trichuris muris, and Romanomermis culicivorax and identified the first putative operons in members of the Enoplea. Consistent with the mechanism of polycistronic RNA resolution in other nematodes, the mRNAs produced by genes downstream of the first gene in the T. spiralis and T. muris operons are trans-spliced to spliced leader RNAs, and we are able to detect polycistronic RNAs derived from these operons. Importantly, a putative intercistronic region from one of these potential enoplean operons confers polycistronic processing activity when expressed as part of a chimeric operon in Caenorhabditis elegans. We find that T. spiralis genes located in operons have an increased likelihood of having operonic C. elegans homologs. However, operon structure in terms of synteny and gene content is not tightly conserved between the two taxa, consistent with models of operon evolution. We have nevertheless identified putative operons conserved between Enoplea and Chromadorea. Our data suggest that operons and “spliced leader” (SL) trans-splicing predate the radiation of the nematode phylum, an inference which is supported by the phylogenetic profile of proteins known to be involved in nematode SL trans-splicing.
登录
查看更多内容
影响因子：
4.5
作者：
Guiliano DB;Blaxter ML
通讯作者：
Blaxter ML
影响因子：
7
作者：
Huang, Peiming;Pleasance, Erin D.;Jones, Steven J. M.
通讯作者：
Jones, Steven J. M.
影响因子：
12.3
作者：
Marletaz, Ferdinand;Gilles, Andre;Caubit, Xavier;Perez, Yvan;Dossat, Carole;Samain, Sylvie;Gyapay, Gabor;Wincker, Patrick;Le Parco, Yannick
通讯作者：
Le Parco, Yannick
影响因子：
14.9
作者：
Dereeper A;Guignon V;Blanc G;Audic S;Buffet S;Chevenet F;Dufayard JF;Guindon S;Lefort V;Lescot M;Claverie JM;Gascuel O
通讯作者：
Gascuel O
影响因子：
7
作者：
Allen, Mary Ann;Hillier, LaDeana W.;Blumenthal, Thomas
通讯作者：
Blumenthal, Thomas