Effects of cycloheximide and actinomycin D on the amino acid transport system of Tetrahymena.
Effects of cycloheximide and actinomycin D on the amino acid transport system of Tetrahymena.
复制标题
放线菌酮和放线菌素 D 对四膜虫氨基酸转运系统的影响。
DOI：
10.1002/jcp.1041110116
复制
发表时间：
1982
影响因子：
5.6
通讯作者：
Blum,JJ
中科院分区：
文献类型：
--
作者：
Blum,JJ
将梨形四膜虫（Tetrahymena pyriformis）无菌培养至对数生长后期和稳定期，重悬于无机培养基中，测定α-氨基异丁酸（AIB）的转运速率以及L-[1 - 14 C]亮氨酸和L-[1 - 14 C]酪氨酸的脱羧速率。有一个快速的损失，每一个这些措施的氨基酸运输在对数晚期和稳定期细胞。此外，放线菌素D的洗涤细胞引起的损失率的能力，脱羧酪氨酸和亮氨酸的一个小的增加。除了放线菌酮的洗涤细胞引起的损失率的能力，运输AIB和脱羧亮氨酸和酪氨酸的减少，在后期对数期细胞放线菌酮显着增加的损失率的能力脱羧亮氨酸。当用氯丙嗪预处理以降低其氨基酸转运能力的细胞被洗涤并重悬于蛋白糖蛋白胨中时，酪氨酸和亮氨酸脱羧的能力在1.5小时内增加至对照值。放线菌素D的加入降低了转运能力的恢复速度，但放线菌酮的加入导致转运能力进一步下降。这些结果提示该细胞可能存在两种氨基酸转运系统。AIB和N-甲基氨基异丁酸盐都被四膜虫吸收，后者的吸收率是前者的八分之一，但两者都没有改变另一种的吸收率，这一发现为这种可能性提供了初步支持。本研究结果进一步表明，转运系统具有较短的寿命，并且转运系统的合成速率和损失速率之间的平衡部分受外源氨基酸的存在控制。
Tetrahymena pyriformiswere grown in axenic culture to late logarithmic and stationary phases, resuspended in an inorganic medium, and the rates of transport of α‐aminoisobutyric acid (AIB) and of the decarboxylation of L‐[1‐14C]leucine and L‐[1‐14C]tyrosine were measured. There was a rapid loss of each of these measures of amino acid transport in both late log phase and stationary phase cells. Addition of actinomycin D to the washed cells caused a small increase in the rate of loss of capacity to decarboxylate tyrosine and leucine. Addition of cycloheximide to the washed cells caused a reduction in the rates of loss of capacity to transport AIB and to decarboxylate leucine and tyrosine except that in late log phase cells cycloheximide markedly increased the rate of loss of capacity to decarboxylate leucine. When cells that had been pretreated with chlorpromazine to reduce their amino acid transport capacity were washed and resuspended in proteose peptone the capacity to decarboxylate tyrosine and leucine increased to control values within 1.5 hours. Addition of actinomycin D reduced the rate of recovery of transport capacity, but addition of cycloheximide caused transport capacity to decrease further. These results raised the possibility that there were two amino acid transport systems in this cell. The finding that AIB and N‐methylaminoisobutyrate are both taken up byTetrahymena, the latter at one‐eighth the rate of the former, but that neither one alters the rate of uptake of the other provides preliminary support for this possibility. The present results further suggest that the transport system(s) has a short lifetime and that the balance between rate of synthesis and rate of loss of the transport system is controlled in part by the presence of exogenous amino acids.
登录
查看更多内容
小鼠肝脏线粒体部分中的环氧化物水解酶活性
影响因子：
64.8
作者：
S. Gill;B. Hammock
通讯作者：
B. Hammock
肝微粒体环氧化物水合酶。
DOI：
10.1016/s0021-9258(17)40311-5
发表时间：
1977
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Y. Lu;D. Jerina;W. Levin
通讯作者：
W. Levin
评估大鼠肝微粒体环氧化物水解酶作为肝癌发生的标志物。
影响因子：
5.8
作者：
D. Kizer;J. A. Clouse;D. Ringer;O. Hanson;A. Vaz;R. Palakodety;M. Griffin
通讯作者：
M. Griffin
人肺中的多种环氧化物水解酶。
DOI：
--
发表时间：
1986
期刊：
Drug metabolism and disposition: the biological fate of chemicals
影响因子：
--
作者：
Guenthner,TM;Karnezis,TA
通讯作者：
Karnezis,TA
大鼠和小鼠中的胞质环氧化物水解酶和微粒体环氧化物水解酶在免疫学上是可区分的。
DOI：
10.1016/s0021-9258(19)69582-7
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Guenthner;B. Hammock;U. Vogel;F. Oesch
通讯作者：
F. Oesch