Effects of calorie restriction on the zebrafish liver proteome.
Effects of calorie restriction on the zebrafish liver proteome.
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DOI：
10.1016/j.cbd.2008.07.003
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发表时间：
2008-12
影响因子：
3
通讯作者：
Londraville, Richard
中科院分区：
文献类型：
--
作者：
Jury, David R.;Kaveti, Surna;Duan, Zhong-Hui;Willard, Belinda;Kinter, Michael;Londraville, Richard
采用蛋白质组学方法研究鱼类如何对卡路里可用性的变化做出反应，其长期目标是了解脊椎动物脂质代谢的演变。斑马鱼（Danio rerio）被喂食高（3份/天）或低（1份/7天）热量饮食5周，并提取肝脏蛋白用于蛋白质组学分析。在双向电泳凝胶上分离蛋白质，并比较处理之间的同源斑点，以确定哪些蛋白质在高热量饮食中上调。从凝胶上切下55个点，并通过LC-ESI MS/MS进行分析，结果鉴定出69种独特的蛋白质（通过多种肽）。这些蛋白质中有29种在治疗之间差异表达。将差异表达的蛋白质映射到基因本体（GO）术语，并通过Fisher精确检验将这些术语与整个斑马鱼GO注释集进行比较。与高热量饮食相关的最重要的GO术语与高热量治疗中氧结合活性的降低有关。这一反应与肥胖人群中的一个特征性反应一致，表明脊椎动物中的脂质储存和缺氧敏感性之间可能存在联系。
A proteomic approach was taken to study how fish respond to changes in calorie availability, with the longer-term goal of understanding the evolution of lipid metabolism in vertebrates. Zebrafish (Danio rerio) were fed either high (3 rations/day) or low (1 ration/7 days) calorie diets for 5 weeks and liver proteins extracted for proteomic analyses. Proteins were separated on two-dimensional electrophoresis gels and homologous spots compared between treatments to determine which proteins were up-regulated with high-calorie diet. Fifty-five spots were excised from the gel and analyzed via LC–ESI MS/MS, which resulted in the identification of 69 unique proteins (via multiple peptides). Twenty-nine of these proteins were differentially expressed between treatments. Differentially expressed proteins were mapped to Gene Ontology (GO) terms, and these terms compared to the entire zebrafish GO annotation set by Fisher's exact test. The most significant GO terms associated with high-calorie diet are related to a decrease in oxygen-binding activity in the high-calorie treatment. This response is consistent with a well-characterized response in obese humans, indicating there may be a link between lipid storage and hypoxia sensitivity in vertebrates.
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影响因子：
--
作者：
LONDRAVILLE, RL;SIDELL, BD
通讯作者：
SIDELL, BD
影响因子：
4.2
作者：
KENNEDY, ML;FAILLA, ML;SMITH, JC
通讯作者：
SMITH, JC
影响因子：
2.9
作者：
Martin, SAM;Cash, P;Houlihan, DF
通讯作者：
Houlihan, DF
影响因子：
4.2
作者：
FAILLA, ML;KENNEDY, ML;CHEN, ML
通讯作者：
CHEN, ML
影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M