A new broccoli × broccoli immortal mapping population and framework genetic map: tools for breeders and complex trait analysis.

A new broccoli × broccoli immortal mapping population and framework genetic map: tools for breeders and complex trait analysis.
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DOI:
10.1007/s00122-011-1721-6
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发表时间:
2012-02
影响因子:
5.4
通讯作者:
Buchanan-Wollaston, Vicky
Buchanan-Wollaston, Vicky
中科院分区:
农林科学1区
文献类型:
--
作者:
Walley, Peter Glen;Carder, John;Skipper, Emma;Mathas, Evy;Lynn, James;Pink, David;Buchanan-Wollaston, Vicky

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利用青花菜双单倍体(DH)品系绿色杜克和马拉松的杂交F1,建立了一个独特的青花菜×青花菜双单倍体(DH)群体。我们用简单重复序列和扩增片段长度多态性标记对该人群中的154名个体进行基因分型,以创建B。oleracea L.变种斜体“作物内”特定框架连锁图。该图谱由9个连锁群组成,总长度为946.7cM。先前发表的B。已经使用B的形态型之间的不同杂交构建了甘蓝图谱。甘蓝;因此,该图谱代表了用于解剖花椰菜特定性状的有用育种资源。表型数据已收集的人口超过五个生长季节的框架连锁图谱已被用来定位数量性状基因座的农艺学上重要的青花菜性状,包括头重(可销售的产量),头直径,茎直径,重量损失和相对重量损失在存储过程中,以及青花菜叶结构的性状。这一群体和相关的连锁图谱将有助于育种者直接绘制农艺学上重要的性状,以改进西兰花的优良品种。本文的在线版本(doi:10.1007/s 00122 -011-1721-6)包含补充材料,可供授权用户使用。
A unique broccoli × broccoli doubled haploid (DH) population has been created from the F1 of a cross between two DH broccoli lines derived from cultivars Green Duke and Marathon. We genotyped 154 individuals from this population with simple sequence repeat and amplified fragment length polymorphism markers to create a B. oleracea L. var. italica ‘intra-crop’ specific framework linkage map. The map is composed of nine linkage groups with a total length of 946.7 cM. Previous published B. oleracea maps have been constructed using diverse crosses between morphotypes of B. oleracea; this map therefore represents a useful breeding resource for the dissection of broccoli specific traits. Phenotype data have been collected from the population over five growing seasons; the framework linkage map has been used to locate quantitative trait loci for agronomically important broccoli traits including head weight (saleable yield), head diameter, stalk diameter, weight loss and relative weight loss during storage, as well as traits for broccoli leaf architecture. This population and associated linkage map will aid breeders to directly map agronomically important traits for the improvement of elite broccoli cultivars. The online version of this article (doi:10.1007/s00122-011-1721-6) contains supplementary material, which is available to authorized users.
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