Characterization of the promoter, downstream target genes and recognition DNA sequence of Mhy1, a key filamentation-promoting transcription factor in the dimorphic yeast Yarrowia lipolytica
Characterization of the promoter, downstream target genes and recognition DNA sequence of Mhy1, a key filamentation-promoting transcription factor in the dimorphic yeast Yarrowia lipolytica
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二态性酵母解脂耶氏酵母中关键的丝化促进转录因子 Mhy1 的启动子、下游靶基因和识别 DNA 序列的表征
DOI：
10.1007/s00294-019-01018-1
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发表时间：
2019-07
期刊：
影响因子：
2.5
通讯作者：
Xiang-Dong Gao
中科院分区：
文献类型：
--
作者：
Heng Wu;Tao Shu;Yi-Sheng Mao;Xiang-Dong Gao
MSN2/MSN4家族锌指转录因子在酵母的应激反应中发挥重要作用。然而，该家族的一些成员在不同的物种中表现出显著的功能差异。在这里，我们报道了在解脂雅罗维菌的二态酵母中，MSN2/MSN4类蛋白Mhy1是酵母到菌丝二态转变的关键调节因子，而不是胁迫反应。MHY1缺失和过表达都会影响细丝形成。相反，另一种类似MSN2/MSN4的蛋白YlMsn4调节对酸诱导的压力的耐受性。我们发现MHY1有一个异常长的启动子(约3800bp)，具有一个位于上游的增强子和一个双应激反应元件(STRE)基序，后者介导MHY1‘S对其自身转录的调控。在野生型菌株、Mhy1Δ突变体和MHY1过表达突变体中进行的转录组图谱显示，与野生型菌株相比，这两个突变体中的每一个都有大约100个基因的高度差异表达(≥ 5倍)。Δ突变体中最大的一组下调基因编码与细胞壁组织有关的细胞壁蛋白或酶，提示Mhy1可能通过控制这些细胞壁基因来调节二态转换。我们证实YALI0C23452、YALI0C15268和YALI0B09955基因受Mhy1直接调控。我们还将Mhy1共识结合位点鉴定为5‘-WNAGGGG-3’(W = A或T；N = A，T，G或C)。这些结果为深入了解真菌中MSN2/MSN4家族转录因子的功能以及Mhy1调控二态转换的机制提供了新的思路。
Msn2/Msn4-family zinc finger transcription factors play important roles in stress response in yeast. However, some members of this family show significant functional divergence in different species. Here, we report that in the dimorphic yeastYarrowia lipolytica, the Msn2/Msn4-like protein Mhy1 is a key regulator of yeast-to-hypha dimorphic transition but not stress response. BothMHY1deletion and overexpression affect filamentation. In contrast, YlMsn4, the other Msn2/Msn4-like protein, regulates tolerance to acid-induced stress. We show thatMHY1has an unusually long (about 3800 bp) promoter featuring an upstream located enhancer and a double stress response element (STRE) motif, the latter of which mediates Mhy1’s regulation on its own transcription. Transcriptome profiling conducted in wild-type strain,mhy1Δ mutant andMHY1-overexpressing mutant revealed about 100 genes that are highly differentially expressed (≥ 5-fold) in each of the 2 mutants compared to the wild-type strain. The largest group of genes downregulated inmhy1Δ mutant encodes cell wall proteins or enzymes involved in cell wall organization, suggesting that Mhy1 may regulate dimorphic transition by controlling these cell wall genes. We confirmed that the genesYALI0C23452,YALI0C15268andYALI0B09955are directly regulated by Mhy1. We also characterized the Mhy1 consensus binding site as 5′-WNAGGGG-3′ (W = A or T; N = A, T, G or C). These results provide new insight in the functions of Msn2/Msn4-family transcription factors in fungi and the mechanism by which Mhy1 regulates dimorphic transition.
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影响因子：
2.5
作者：
Yingzi Yun;Xin Zhou;Shuai Yang;Y. Wen;Haixia You;Yuru Zheng;Justice Norvienyeku;W. Shim;Zonghua Wang
通讯作者：
Yingzi Yun;Xin Zhou;Shuai Yang;Y. Wen;Haixia You;Yuru Zheng;Justice Norvienyeku;W. Shim;Zonghua Wang
影响因子：
3.2
作者：
X. Zhao;Min Li;Yun-qing Li;Xiang-Dong Chen;Xiang-Dong Gao
通讯作者：
X. Zhao;Min Li;Yun-qing Li;Xiang-Dong Chen;Xiang-Dong Gao
影响因子：
3.6
作者：
Leberer, E;Harcus, D;Schröppel, K
通讯作者：
Schröppel, K
影响因子：
64.8
作者：
Dujon, B;Sherman, D;Souciet, JL
通讯作者：
Souciet, JL
影响因子：
6.7
作者：
Kadosh D
通讯作者：
Kadosh D