Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle.
Global transcriptome analysis identifies differentially expressed genes related to lipid metabolism in Wagyu and Holstein cattle.
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DOI：
10.1038/s41598-017-05702-5
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发表时间：
2017-07-13
影响因子：
4.6
通讯作者：
Miao X
中科院分区：
文献类型：
--
作者：
Huang W;Guo Y;Du W;Zhang X;Li A;Miao X
肉牛的脂肪沉积因品种而异。然而，其调控机制在分子水平上仍未完全阐明。在本研究中，我们比较分析了脂肪沉积差异显着的和牛和荷斯坦牛皮下脂肪组织的转录组，通过高通量RNA-seq技术鉴定与脂肪代谢和脂肪形成相关的关键基因。总共分别生成了 59,149,852 和 69,947,982 条高质量读数。通过进一步分析，鉴定出662个差异表达基因。 Gene Ontology和KEGG通路分析显示，许多差异表达基因富集在与脂肪形成和脂质代谢相关的多个生物过程和通路中，其中PPAR和脂肪酸代谢信号通路与相关基因如PPARγ、PLIN2和ELOVL6等相关。发挥关键作用。蛋白质-蛋白质相互作用网络分析显示EGR1、FOS、SERPINE1、AGT、MMP2可能对脂肪细胞分化和脂肪形成有很大影响。此外，还鉴定了潜在的选择性剪接事件和单核苷酸多态性（SNP）。综上所述，我们对和牛和荷斯坦牛皮下脂肪组织的转录组进行了全面的分析和讨论，为更好地理解肉牛脂肪代谢和沉积的分子机制提供理论基础。
Fat deposition of beef cattle varies between breeds. However, the regulation mechanism is still not elucidated completely at molecular level. In the present study, we comparatively analyzed transcriptome of subcutaneous adipose tissue between Wagyu and Holstein cattle with a significant difference in fat deposition to identify key genes associated with fat metabolism and adipogenesis by high-throughput RNA-seq technology. A total of 59,149,852 and 69,947,982 high quality reads were generated, respectively. With further analysis, 662 differentially expressed genes were identified. Gene Ontology and KEGG pathway analysis revealed that many differentially expressed genes were enriched in several biological processes and pathways relevant to adipogenesis and lipid metabolism, in which PPAR and fatty acid metabolism signaling pathways with related genes such as PPARγ, PLIN2 and ELOVL6 et al. play a critical role. Protein-protein interaction network analysis showed EGR1, FOS, SERPINE1, AGT, MMP2 may have great impact on adipocyte differentiation and adipogenesis. Moreover, potential alternative splicing events and single nucleotide polymorphisms (SNPs) were also identified. In summary, we comprehensively analyzed and discussed the transcriptome of subcutaneous adipose tissue of Wagyu and Holstein cattle, which might provide a theoretical basis for better understanding molecular mechanism of fat metabolism and deposition in beef cattle.
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影响因子：
2.9
作者：
Bionaz M;Chen S;Khan MJ;Loor JJ
通讯作者：
Loor JJ
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
5.6
作者：
Li N;Zhao F;Wei C;Liang M;Zhang N;Wang C;Li QZ;Gao XJ
通讯作者：
Gao XJ
影响因子：
5.2
作者：
Carroll WX;Kalupahana NS;Booker SL;Siriwardhana N;Lemieux M;Saxton AM;Moustaid-Moussa N
通讯作者：
Moustaid-Moussa N