Chronic exposure to the star polycation (SPc) nanocarrier in the larval stage adversely impairs life history traits in Drosophila melanogaster.
Chronic exposure to the star polycation (SPc) nanocarrier in the larval stage adversely impairs life history traits in Drosophila melanogaster.
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DOI：
10.1186/s12951-022-01705-1
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发表时间：
2022-12-08
影响因子：
10.2
通讯作者：
Zhang, Junzheng
中科院分区：
文献类型：
--
作者：
Yan, Shuo;Li, Na;Guo, Yuankang;Chen, Yao;Ji, Chendong;Yin, Meizhen;Shen, Jie;Zhang, Junzheng
纳米材料被广泛用作农药助剂，以提高农药效率并减少环境污染。但随着纳米颗粒作为新的环境污染物的出现，人们越来越认识到纳米载体是一把双刃剑。本研究旨在以果蝇为体内模型，测定一种广泛应用的星星聚阳离子（SPc）纳米载体的生物毒性。对3龄幼虫和成虫的LC 50分别为2.14 g/L和26.33 g/L。在幼虫阶段长期暴露于亚致死浓度的SPc（1克/升）对关键的生活史特征表现出持久的不利影响。幼虫期暴露于SPc会对羽化成虫的寿命、生育力、攀援能力以及抗逆性产生不利影响。RNA测序分析发现，SPc导致参与代谢、先天免疫、应激反应和幼虫激素产生的基因的异常表达。口服给药的SPc纳米粒主要在肠道细胞中积累，而观察到全身反应。这些发现表明，SPc纳米颗粒对果蝇有多个层次的危害，这可以帮助我们制定进一步大规模应用的指导方针。在线版本包含补充材料，可通过10.1186/s12951-022-01705-1获得。
Nanomaterials are widely used as pesticide adjuvants to increase pesticide efficiency and minimize environmental pollution. But it is increasingly recognized that nanocarrier is a double-edged sword, as nanoparticles are emerging as new environmental pollutants. This study aimed to determine the biotoxicity of a widely applied star polycation (SPc) nanocarrier using Drosophila melanogaster, the fruit fly, as an in vivo model. The lethal concentration 50 (LC50) value of SPc was identified as 2.14 g/L toward third-instar larvae and 26.33 g/L for adults. Chronic exposure to a sub lethal concentration of SPc (1 g/L) in the larval stage showed long-lasting adverse effects on key life history traits. Exposure to SPc at larval stage adversely impacted the lifespan, fertility, climbing ability as well as stresses resistance of emerged adults. RNA-sequencing analysis found that SPc resulted in aberrant expression of genes involved in metabolism, innate immunity, stress response and hormone production in the larvae. Orally administrated SPc nanoparticles were mainly accumulated in intestine cells, while systemic responses were observed. These findings indicate that SPc nanoparticles are hazardous to fruit flies at multiple levels, which could help us to develop guidelines for further large-scale application. The online version contains supplementary material available at 10.1186/s12951-022-01705-1.
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