Modulatory Effects of Lactobacillus salivarius on Intestinal Mucosal Immunity of Piglets
Modulatory Effects of Lactobacillus salivarius on Intestinal Mucosal Immunity of Piglets
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唾液乳杆菌对仔猪肠粘膜免疫的调节作用
DOI：
10.1007/s00284-011-9906-4
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发表时间：
2011-03
影响因子：
2.6
通讯作者：
Deng, Jun
中科院分区：
文献类型：
--
作者：
Che, Chuanyan;Wang, Zhisheng;Li, Yun-feng;Zhang, Jinhua;Yang, Qian;Deng, Jun
最近的研究表明，乳酸杆菌或其细胞成分可以提高动物的某些免疫功能。本研究旨在探讨猪乳酸杆菌对仔猪肠道黏膜免疫功能的影响。以新生仔猪为模型，经口给予从健康仔猪十二指肠粘膜分离的唾液乳杆菌B1。在第7、14和21天收集仔猪的粪便用于肠道微生物菌群分析。第28天处死仔猪，立即收集其肠粘膜样品，以研究肠道形态学和免疫活性细胞的变化。最后，检测细胞因子和TLRs在不同肠段的表达。结果表明，L.唾液B1可部分改善肠道菌群，增加肠道免疫活性细胞数量，与对照组相比，乳酸菌处理组的上皮内淋巴细胞（P < 0.05）和IgA产生细胞（P < 0.01）均增加。回肠中IL-6基因表达增强（P < 0.05）。此外，L. salivarius B1还能在基因和蛋白水平上调肠道TLR 2的表达（P < 0.05）。结果表明，L.唾液B1有利于肠粘膜免疫系统的成熟，并引起局部免疫调节活性。此外，L. salivariusB 1对粘膜免疫的作用主要依赖于其胞外成分。
Recent studies have demonstrated that lactobacilli or their cell components can improve certain immune function in animals. The aim of this study is to investigate the effects of porcine lactobacilli on the intestinal mucosal immunity of piglets. Neonatal piglets were used as a model and were orally administrated with Lactobacillus salivarius B1 isolated from the duodenal mucosa of a healthy piglet. The feces of the piglets were collected on days 7, 14, and 21 for intestinal microflora analysis. On day 28, the piglets were sacrificed, and their intestinal mucosa samples were immediately collected to investigate the changes in intestinal morphological and immunocompetent cells. Finally, the expression of cytokines and TLRs was detected in the different intestinal segments. The results indicate that L. salivarius B1 can partially ameliorate the microflora of the feces and increase the number of intestinal immunocompetent cells, as the intraepithelial lymphocyte (P < 0.05), and the IgA-producing cells (P < 0.01) in the lactobacilli-treated group were all increased compared with those in the control group. Enhanced expression of the cytokine IL-6 gene was also observed in the ileum (P < 0.05). Moreover, L. salivarius B1 can also upregulate the expression of TLR2 in the intestinal tract at the gene and protein levels (P < 0.05). The results demonstrate that L. salivarius B1 is beneficial for the maturation of the intestinal mucosal immune system and elicited local immunomodulatory activities. In addition, the modulatory effects of L. salivarius B1 on mucosal immunity mainly depend on its extracellular components.
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影响因子：
8
作者：
通讯作者：
--
影响因子：
3.7
作者：
Che, C.;Pang, X.;Yang, Q.
通讯作者：
Yang, Q.
影响因子：
2.2
作者：
Liu, Fangning;Li, Guohua;Yuan, Lijuan
通讯作者：
Yuan, Lijuan
影响因子：
6.7
作者：
Nishimoto, N.
通讯作者：
Nishimoto, N.
影响因子：
--
作者：
A. Macpherson
通讯作者：
A. Macpherson