Evolution of a Cytoplasmic Determinant: Evidence for the Biochemical Basis of Functional Evolution of the Novel Germ Line Regulator Oskar.
Evolution of a Cytoplasmic Determinant: Evidence for the Biochemical Basis of Functional Evolution of the Novel Germ Line Regulator Oskar.
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DOI：
10.1093/molbev/msab284
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发表时间：
2021-12-09
影响因子：
10.7
通讯作者：
Extavour CG
中科院分区：
文献类型：
--
作者：
Blondel L;Besse S;Rivard EL;Ylla G;Extavour CG
生殖系的特化在有性繁殖的生物体中是必不可少的。尽管它们的关键作用，指定动物生殖细胞的基因的进化历史是异质的和动态的。在许多昆虫中，oskar基因是生殖细胞系特化所必需的。然而，oskar的生殖系作用被认为是一种衍生作用，来自祖先体细胞作用的协同选择。为了解决蛋白质序列的进化变化如何导致Oskar蛋白功能的变化，使其能够调节生殖系特化，我们在1，565个公开的昆虫基因组和转录组数据集中搜索了Oskar直系同源物。最早的分歧谱系中，我们确定了一个奥斯卡的直系同源物是Zygentoma（银鱼和firebrats），这表明奥斯卡起源于有翼昆虫的起源之前。我们注意到在oskar序列进化中的一些特定目的趋势，包括全基因复制，分支特定的损失和快速分化。所有已知的379个Oskar序列的比对揭示了新的高度保守的残基作为促进LOTUS结构域二聚化的候选物。此外，我们确定了保守的预测RNA结合潜力的OSK域的区域。此外，我们发现，尽管低的整体氨基酸保守性，LOTUS域显示出较高的保守性预测的二级结构比OSK域。最后，我们提出了LOTUS结构域中新的关键氨基酸，这些氨基酸可能参与了先前报道的Oskar−Vasa物理相互作用，这是其生殖细胞作用所必需的。
Germ line specification is essential in sexually reproducing organisms. Despite their critical role, the evolutionary history of the genes that specify animal germ cells is heterogeneous and dynamic. In many insects, the gene oskar is required for the specification of the germ line. However, the germ line role of oskar is thought to be a derived role resulting from co-option from an ancestral somatic role. To address how evolutionary changes in protein sequence could have led to changes in the function of Oskar protein that enabled it to regulate germ line specification, we searched for oskar orthologs in 1,565 publicly available insect genomic and transcriptomic data sets. The earliest-diverging lineage in which we identified an oskar ortholog was the order Zygentoma (silverfish and firebrats), suggesting that oskar originated before the origin of winged insects. We noted some order-specific trends in oskar sequence evolution, including whole gene duplications, clade-specific losses, and rapid divergence. An alignment of all known 379 Oskar sequences revealed new highly conserved residues as candidates that promote dimerization of the LOTUS domain. Moreover, we identified regions of the OSK domain with conserved predicted RNA binding potential. Furthermore, we show that despite a low overall amino acid conservation, the LOTUS domain shows higher conservation of predicted secondary structure than the OSK domain. Finally, we suggest new key amino acids in the LOTUS domain that may be involved in the previously reported Oskar−Vasa physical interaction that is required for its germ line role.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
10.5
作者：
Breitwieser, W;Markussen, FH;Ephrussi, A
通讯作者：
Ephrussi, A
影响因子：
3.7
作者：
Carter, Jean-Michel;Gibbs, Melanie;Breuker, Casper J.
通讯作者：
Breuker, Casper J.
影响因子：
14.9
作者：
Drozdetskiy A;Cole C;Procter J;Barton GJ
通讯作者：
Barton GJ
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T