Effect of macrophage classical (M1) activation on implant-adherent macrophage interactions with Staphylococcus epidermidis: A murine in vitro model system.
Effect of macrophage classical (M1) activation on implant-adherent macrophage interactions with Staphylococcus epidermidis: A murine in vitro model system.
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DOI：
10.1002/jbm.a.34087
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发表时间：
2012-08
影响因子：
4.9
通讯作者：
Bryers, James D.
中科院分区：
文献类型：
--
作者：
Park, Kyung R.;Bryers, James D.
开发了一种体外系统模型，用于引发表面粘附的鼠巨噬细胞的经典（M1）激活，然后用于研究 M1 巨噬细胞与表皮葡萄球菌的相互作用。首先用甲氧基和生物素封端的硅烷化聚乙二醇的混合物共价接枝玻璃基质。干扰素 (IFN)-γ 和/或脂多糖 (LPS) 是已知可在巨噬细胞中诱导高度杀菌 M1 激活状态的配体，它们被生物素化并通过链霉亲和素中间体固定到生物素-PEG 基础基质上。对小鼠骨髓源性巨噬细胞 (BMDM) 白细胞介素 (IL)-12(p40) 和一氧化氮对制造表面的反应的评估证实，模型系统实现了贴壁巨噬细胞的激活：IFN-γ 呈递表面引发细胞 M1 激活，LPS 呈递表面引发先天激活，表面呈递 IFN-γ 和 LPS 组合诱导 M1 激活。使用表皮葡萄球菌（一种在植入物相关感染中普遍存在的细菌）评估了活化的表面粘附 BMDM 的吞噬和杀菌能力。结果表明，M1 激活植入物粘附的巨噬细胞会降低其吞噬能力，但会增强其对表皮葡萄球菌的杀菌能力。
A model in vitro system was developed for eliciting classical (M1) activation of surface-adherent murine macrophages, which was then used to study the interaction of the M1 macrophages with Staphylococcus epidermidis. Glass substrata were first covalently grafted with a mixture of methoxy- and biotin-terminated silanated polyethylene glycol. Interferon (IFN)-γ and/or lipopolysaccharide (LPS), ligands known to induce the highly microbicidal M1 activation state in macrophages, were biotinylated and immobilized by way of a streptavidin intermediate to the biotin-PEG base substratum. Assessment of mouse bone marrow-derived macrophage (BMDM) interleukin (IL)-12(p40) and nitric oxide response to the fabricated surfaces confirmed that the model system achieved activation of adherent macrophage: IFN-γ-presenting surfaces primed cells for M1 activation, LPS-presenting surfaces elicited innate activation, and surface presenting a combination of IFN-γ and LPS induced M1 activation. The phagocytic and microbicidal capacity of activated, surface-adherent BMDM was evaluated using S. epidermidis, a bacterial species prevalent in implant-associated infections. Results indicate that M1 activation of implant-adherent macrophages trends towards diminishing their phagocytic capacity, but enhances their microbicidal capacity for S. epidermidis.
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影响因子：
4.9
作者：
Schmidt, David Richard;Kao, Weiyuan John
通讯作者：
Kao, Weiyuan John
影响因子：
2.9
作者：
Luk, JM;Kumar, A;Staunton, D
通讯作者：
Staunton, D
影响因子：
3.1
作者：
Martinez, Fernando Oneissi;Sica, Antonio;Locati, Massimo
通讯作者：
Locati, Massimo
DOI：
10.1016/j.cimid.2009.07.001
发表时间：
2010-09
期刊：
Comparative immunology, microbiology and infectious diseases
影响因子：
--
作者：
Berghaus LJ;Moore JN;Hurley DJ;Vandenplas ML;Fortes BP;Wolfert MA;Boons GJ
通讯作者：
Boons GJ
影响因子：
4.4
作者：
Stout, RD;Jiang, CC;Suttles, J
通讯作者：
Suttles, J