The potential of label-free nonlinear optical molecular microscopy to non-invasively characterize the viability of engineered human tissue constructs.
The potential of label-free nonlinear optical molecular microscopy to non-invasively characterize the viability of engineered human tissue constructs.
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DOI：
10.1016/j.biomaterials.2014.04.080
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发表时间：
2014-08
期刊：
影响因子：
14
通讯作者：
Mycek, Mary-Ann
中科院分区：
文献类型：
--
作者：
Chen, Leng-Chun;Lloyd, William R.;Kuo, Shiuhyang;Kim, Hyungjin Myra;Marcelo, Cynthia L.;Feinberg, Stephen E.;Mycek, Mary-Ann
关键词：
发展了非线性光学分子成像和定量分析方法，以非侵入性地评估由原代人类细胞制造的组织工程构建物的活性。局部组织结构和生化的无标记光学测量表征了对照和应激结构之间的形态和功能差异。严格的统计分析解释了人类患者之间的差异。基于荧光强度的空间评估和代谢感知将对照与热应激和新陈代谢应激结构区分开来。基于荧光寿命的传感区分了对照和热应激构建物。与传统的组织学(通常被发现是可靠的，但具有破坏性)和生化(非侵入性，但被发现不可靠)组织分析不同，无标记光学评估具有非侵入性和可靠性的优点。因此，这样的光学测量可以作为基于细胞的组织工程结构在人类植入之前的可靠制造释放标准，从而解决再生医学中的关键监管需求。
Nonlinear optical molecular imaging and quantitative analytic methods were developed to non-invasively assess the viability of tissue-engineered constructs manufactured from primary human cells. Label-free optical measures of local tissue structure and biochemistry characterized morphologic and functional differences between controls and stressed constructs. Rigorous statistical analysis accounted for variability between human patients. Fluorescence intensity-based spatial assessment and metabolic sensing differentiated controls from thermally-stressed and from metabolically-stressed constructs. Fluorescence lifetime-based sensing differentiated controls from thermally-stressed constructs. Unlike traditional histological (found to be generally reliable, but destructive) and biochemical (non-invasive, but found to be unreliable) tissue analyses, label-free optical assessments had the advantages of being both non-invasive and reliable. Thus, such optical measures could serve as reliable manufacturing release criteria for cell-based tissue-engineered constructs prior to human implantation, thereby addressing a critical regulatory need in regenerative medicine.
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