Quantitative iTRAQ LC-MS/MS proteomics reveals the proteome profiles of DF-1 cells after infection with subgroup J Avian leukosis virus.
Quantitative iTRAQ LC-MS/MS proteomics reveals the proteome profiles of DF-1 cells after infection with subgroup J Avian leukosis virus.
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定量 iTRAQ LC-MS/MS 蛋白质组学揭示了 J 亚型禽白血病病毒感染后 DF-1 细胞的蛋白质组图谱
DOI：
10.1155/2015/395307
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发表时间：
2015
影响因子：
--
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Li X;Wang Q;Gao Y;Qi X;Wang Y;Gao H;Gao Y;Wang X
Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that can induce various clinical tumors and has caused severe economic losses in China. To improve our understanding of the host cellular responses to virus infection and the pathogenesis of ALV-J infection, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with multidimensional liquid chromatography-tandem mass spectrometry to detect the protein changes in DF-1 cells infected and mock-infected with ALV-J. A total of 75 cellular proteins were significantly changed, including 33 upregulated proteins and 42 downregulated proteins. The reliability of iTRAQ-LC MS/MS was confirmed via real-time PCR. Most of these proteins were related to the physiological functions of metabolic processes, biosynthetic processes, responses to stimuli, protein binding, signal transduction, cell cytoskeleton, and so forth. We also found some proteins that play important roles in apoptosis and oncogenicity. The differentially expressed proteins identified may provide valuable information to elucidate the pathogenesis of virus infection and virus-host interactions.
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DOI：
10.1016/j.bbrc.2007.06.094
发表时间：
2007-08-31
影响因子：
3.1
作者：
Oka, Naoki;Soeda, Akio;Iwama, Toru
通讯作者：
Iwama, Toru
影响因子：
4.4
作者：
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通讯作者：
Jiang, Ping
影响因子：
8
作者：
Catalano, A;Romano, M;Procopio, A
通讯作者：
Procopio, A
影响因子：
8
作者：
Lorenzon, E.;Colladel, R.;Mongiat, M.
通讯作者：
Mongiat, M.
影响因子：
1.4
作者：
PAYNE, LN;GILLESPIE, AM;HOWES, K
通讯作者：
HOWES, K