Map location of lactate dehydrogenase-elevating virus (LDV) capsid protein (Vp1) gene.
Map location of lactate dehydrogenase-elevating virus (LDV) capsid protein (Vp1) gene.
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DOI：
10.1016/0042-6822(90)90546-4
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发表时间：
1990-08
期刊：
影响因子：
3.7
通讯作者：
Brinton MA
中科院分区：
文献类型：
--
作者：
Godeny EK;Speicher DW;Brinton MA
乳酸脱氢酶升高病毒（LDV）目前被归类为托加病毒科。为了进一步了解这种病毒的特征，我们已经开始对病毒RNA基因组进行测序，并绘制病毒粒子结构蛋白基因图谱。从LDV cDNA克隆中获得了1064个核苷酸序列，代表了基因组的3 '末端。在该序列中发现了一个包含80个核苷酸的3 '非编码区，其后是两个完整的开放阅读框（orf）。这两个orf位于不同的阅读框中，彼此重叠了11个核苷酸。一个ORF编码170个氨基酸的蛋白质，另一个ORF位于病毒基因组3 '非编码区附近，编码114个氨基酸的蛋白质。从纯化的LDV衣壳蛋白Vpi中直接测序了33个n端残基，该氨基酸序列与3 '非编码区相邻的ORF相对应。重叠orf的存在和Vpi的3′末端图谱位置表明LDV与原型α托加病毒有显著差异。
Lactate dehydrogenase-elevating virus (LDV) is currently classified within the Togaviridae family. In an effort to obtain further information on the characteristics of this virus, we have begun to sequence the viral RNA genome and to map the virion structural protein genes. A sequence of 1064 nucleotides, which represents the 3′ terminal end of the genome, was obtained from LDV cDNA clones. A 3′ noncoding region of 80 nucleotides followed by two complete open reading frames (ORFs) were found within this sequence. The two ORFs were in different reading frames and overlapped each other by 11 nucleotides. One ORF encoded a protein of 170 amino acids and the other ORF, located adjacent to the 3′ noncoding region of the viral genome, encoded a 114 amino acid protein. Thirty-three N-terminal residues were sequenced directly from purified LDV capsid protein, Vpi, and this amino acid sequence mapped to the ORF adjacent to the 3′ noncoding region. The presence of overlapping ORFs and the 3′ terminal map position of Vpi indicate that LDV differs significantly from the prototype alpha togaviruses.
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影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
影响因子：
3.1
作者：
MARTINEZ, D;BRINTON, MA;PHELPS, AH
通讯作者：
PHELPS, AH
DOI：
10.1073/pnas.76.9.4350
发表时间：
1979-01-01
影响因子：
11.1
作者：
TOWBIN, H;STAEHELIN, T;GORDON, J
通讯作者：
GORDON, J
影响因子：
3.8
作者：
BRINTON, MA;GAVIN, EI;FERNANDEZ, AV
通讯作者：
FERNANDEZ, AV
影响因子：
3.8
作者：
HORZINEK, MC;VANWIELINK, PS;ELLENS, DJ
通讯作者：
ELLENS, DJ