Imaging of alcohol-induced dopamine release in rats:preliminary findings with [(11) C]raclopride PET.
Imaging of alcohol-induced dopamine release in rats:preliminary findings with [(11) C]raclopride PET.
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DOI：
10.1002/syn.20921
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发表时间：
2011-09
期刊：
影响因子：
2.3
通讯作者：
Morris, Evan D.
中科院分区：
文献类型：
--
作者：
Sullivan, Jenna M.;Risacher, Shannon L.;Normandin, Marc D.;Yoder, Karmen K.;Froehlich, Janice C.;Morris, Evan D.
微透析研究报告，全身酒精增加细胞外多巴胺在大鼠纹状体。本研究探讨是否可以检测到纹状体多巴胺的变化在大鼠使用小动物正电子发射断层扫描（PET）。PET图像采集在44个酒精初治的雄性Wistar和酒精偏好（P）大鼠。受试者接受最多3次[11 C]雷氯必利扫描（休息、酒精和生理盐水）。在所有扫描过程中，用异氟烷麻醉动物，并将其固定在立体定向样保持器上。12只动物在示踪剂注射后10分钟从尾静脉或侧隐静脉采集血液样品，用于测定血液酒精浓度（BAC）。从纹状体和小脑提取时间活性曲线，并计算结合电位（BPND）作为D2受体可用性的量度。静脉注射1.0g/kg酒精（20%v/v）或腹腔注射3.0g/kg酒精（20%v/v）可显著降低酒精引起的BPND。在P大鼠（给予1.5，2.25或3.0 g/kg酒精）中，没有单独的组显示出酒精对BPND的统计学影响，但总的来说，所有接受IP的P大鼠酒精的BPND显著低于休息或盐水扫描。BPND的大幅下降主要见于BAC高于200 mg%的大鼠。此外，在接受颈静脉导管插入术静脉注射酒精的Wistars患者和未接受颈静脉导管插入术静脉注射酒精的Wistars患者的基线BPND之间存在显著差异。这些初步结果表明，酒精诱导的DA释放在大鼠纹状体是检测使用小动物PET给予足够大的队列和足够的血液酒精水平。
Microdialysis studies report that systemic alcohol increases extracellular dopamine in the rat striatum. The present study examined whether changes in striatal dopamine could be detected in rats using small animal positron emission tomography (PET). PET images were acquired in 44 alcohol-naïve male Wistar and alcohol-preferring (P) rats. Subjects received up to three [11C]raclopride scans (rest, alcohol, and saline). Animals were anesthetized with isoflurane and secured on a stereotactic-like holder during all scans. Blood samples were collected from the tail or lateral saphenous vein of 12 animals 10 minutes after tracer injection for determination of blood alcohol concentration (BAC). Time activity curves were extracted from the striatum and the cerebellum and binding potential (BPND) was calculated as a measure of D2 receptor availability. Wistars given 1.0g/kg alcohol (20%v/v) I.V. or 3.0g/kg alcohol (20%v/v) I.P showed significant alcohol-induced decreases in BPND. In P rats (given 1.5, 2.25, or 3.0 g/kg alcohol), no individual group showed a statistical effect of alcohol on BPND, but taken together, all P rats receiving I.P. alcohol had significantly lower BPND than rest or saline scans. Large decreases in BPND were primarily observed in rats with BAC above 200 mg%. Also, a significant difference was found between baseline BPND of Wistars who had undergone jugular catheterization surgery for I.V. alcohol administration and those who had not. These preliminary results suggest that alcohol-induced DA release in the rat striatum is detectable using small animal PET given sufficiently large cohorts and adequate blood alcohol levels.
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影响因子：
3
作者：
Cheng, Tee Ean;Yoder, Karmen K.;Morris, Evan D.
通讯作者：
Morris, Evan D.
影响因子：
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De Montis, MG
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通讯作者：
VITKUN, S
影响因子：
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作者：
Kurtz, DL;Stewart, RB;Froehlich, JC
通讯作者：
Froehlich, JC