Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans.
Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans.
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有机酸对产油酵母发酵丝孢菌生长和脂质积累的影响
DOI：
10.1186/1754-6834-5-4
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发表时间：
2012-01-19
影响因子：
6.3
通讯作者：
Zong MH
中科院分区：
文献类型：
--
作者：
Huang C;Wu H;Liu ZJ;Cai J;Lou WY;Zong MH
背景
微生物油脂作为生物柴油生产的重要原料，近年来受到越来越多的关注，而利用木质纤维素酶解物作为碳源，似乎是大规模利用油脂微生物进行低成本油脂发酵的可行策略。然而，木质纤维原料在稀酸水解过程中会产生各种抑制物，尤其是大量的有机酸，大大降低了木质纤维素水解物的发酵性。为了克服有机酸的抑制作用，了解有机酸对油脂微生物生长和脂肪积累的影响是至关重要的。
结果
在本工作中，我们首次研究了木质纤维素水解液中的10种代表性有机酸对产油酵母发酵毛孢子菌生长和脂肪积累的影响。与以往的报道相比，我们发现有机酸对细胞的毒性与其疏水性没有直接关系。值得注意的是，所测试的大多数有机酸对细胞的毒性都低于醛，有些甚至在低浓度时就能刺激细胞生长和脂质积累。与醛不同的是，大多数有机酸的二元组合对脂肪的产生没有协同抑制作用。有机酸的存在减缓了葡萄糖的消耗，但对木糖的利用有不同而复杂的影响。此外，除呋喃甲酸外，所有供试有机酸均对发酵葡萄球菌的苹果酸活性有抑制作用。此外，有机酸对细胞生长的抑制作用更多地依赖于接种量、温度和初始pH，而不是脂肪含量。
结论
本工作为研究木质纤维素水解液中有机酸对产油酵母产油的影响提供了有意义的信息，为优化生物质水解液的工艺条件、水解物的脱毒前处理以及利用木质纤维原料生产油脂提供了有益的参考。
Background
Microbial lipids have drawn increasing attention in recent years as promising raw materials for biodiesel production, and the use of lignocellulosic hydrolysates as carbon sources seems to be a feasible strategy for cost-effective lipid fermentation with oleaginous microorganisms on a large scale. During the hydrolysis of lignocellulosic materials with dilute acid, however, various kinds of inhibitors, especially large amounts of organic acids, will be produced, which substantially decrease the fermentability of lignocellulosic hydrolysates. To overcome the inhibitory effects of organic acids, it is critical to understand their impact on the growth and lipid accumulation of oleaginous microorganisms.
Results
In our present work, we investigated for the first time the effect of ten representative organic acids in lignocellulosic hydrolysates on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans cells. In contrast to previous reports, we found that the toxicity of the organic acids to the cells was not directly related to their hydrophobicity. It is worth noting that most organic acids tested were less toxic than aldehydes to the cells, and some could even stimulate the growth and lipid accumulation at a low concentration. Unlike aldehydes, most binary combinations of organic acids exerted no synergistic inhibitory effects on lipid production. The presence of organic acids decelerated the consumption of glucose, whereas it influenced the utilization of xylose in a different and complicated way. In addition, all the organic acids tested, except furoic acid, inhibited the malic activity of T. fermentans. Furthermore, the inhibition of organic acids on cell growth was dependent more on inoculum size, temperature and initial pH than on lipid content.
Conclusions
This work provides some meaningful information about the effect of organic acid in lignocellulosic hydrolysates on the lipid production of oleaginous yeast, which is helpful for optimization of biomass hydrolysis processes, detoxified pretreatment of hydrolysates and lipid production using lignocellulosic materials.
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影响因子：
11.4
作者：
Papamkolaou, Seraphim;Galiotou-Panayotou, Maria;Aggelis, George
通讯作者：
Aggelis, George
影响因子：
2.7
作者：
TRAN, AV;CHAMBERS, RP
通讯作者：
CHAMBERS, RP
影响因子：
2.9
作者：
Oliva, JM;Ballesteros, I;Ballesteros, M
通讯作者：
Ballesteros, M
影响因子：
4.4
作者：
Mussatto, SI;Dragone, G;Roberto, IC
通讯作者：
Roberto, IC
影响因子：
3
作者：
Duarte, LC;Carvalheiro, F;Gírio, FM
通讯作者：
Gírio, FM