LED Optrode with Integrated Temperature Sensing for Optogenetics.
LED Optrode with Integrated Temperature Sensing for Optogenetics.
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DOI：
10.3390/mi9090473
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发表时间：
2018-09-17
期刊：
影响因子：
3.4
通讯作者：
Correia JH
中科院分区：
文献类型：
--
作者：
Goncalves SB;Palha JM;Fernandes HC;Souto MR;Pimenta S;Dong T;Yang Z;Ribeiro JF;Correia JH
在光遗传学研究中，大脑暴露于高功率光源，功率密度或暴露时间不足会导致过热（通常温度升高2 ℃）造成细胞损伤。为了克服光遗传学中的过热问题，本文提出了一种能够随着时间的推移评估组织温度的神经工具，结合电记录和光刺激的能力。一个硅基8毫米长的探针被制造出来，以达到深层神经结构。最终的概念验证设备包括双面功能：在一侧，具有基于LED的刺激和铂（Pt）记录点的光极;以及在相对侧，用于在光刺激部位周围环境中进行温度评估的基于Pt的薄膜热电阻（RTD）。由于铂薄膜具有良好的生物相容性和热线性，因此选择铂薄膜作为组织界面材料。演示了用于集成在3D探针阵列中的单轴探针。3D探头阵列将减少热传感器和加热源之间的距离。结果表明，该薄膜具有良好的记录和光学特性，在1kHz处的平均阻抗值为371K，在470nm处的光功率为1.2mW·mm。制造的RTD在37 ℃（正常体温）下显示出0.2 ℃的分辨率。总的来说，结果显示了能够满足用于记录/刺激神经活动和监测光刺激部位周围环境的温度分布的神经接口的要求的设备，这表明神经科学研究领域的有前途的工具。
In optogenetic studies, the brain is exposed to high-power light sources and inadequate power density or exposure time can cause cell damage from overheating (typically temperature increasing of 2 C). In order to overcome overheating issues in optogenetics, this paper presents a neural tool capable of assessing tissue temperature over time, combined with the capability of electrical recording and optical stimulation. A silicon-based 8 mm long probe was manufactured to reach deep neural structures. The final proof-of-concept device comprises a double-sided function: on one side, an optrode with LED-based stimulation and platinum (Pt) recording points; and, on the opposite side, a Pt-based thin-film thermoresistance (RTD) for temperature assessing in the photostimulation site surroundings. Pt thin-films for tissue interface were chosen due to its biocompatibility and thermal linearity. A single-shaft probe is demonstrated for integration in a 3D probe array. A 3D probe array will reduce the distance between the thermal sensor and the heating source. Results show good recording and optical features, with average impedance magnitude of 371 k, at 1 kHz, and optical power of 1.2 mW·mm (at 470 nm), respectively. The manufactured RTD showed resolution of 0.2 C at 37 C (normal body temperature). Overall, the results show a device capable of meeting the requirements of a neural interface for recording/stimulating of neural activity and monitoring temperature profile of the photostimulation site surroundings, which suggests a promising tool for neuroscience research filed.
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DOI：
10.1126/science.1232437
发表时间：
2013-04-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kim TI;McCall JG;Jung YH;Huang X;Siuda ER;Li Y;Song J;Song YM;Pao HA;Kim RH;Lu C;Lee SD;Song IS;Shin G;Al-Hasani R;Kim S;Tan MP;Huang Y;Omenetto FG;Rogers JA;Bruchas MR
通讯作者：
Bruchas MR
DOI：
10.1109/tbcas.2013.2282318
发表时间：
2013-10-01
影响因子：
5.1
作者：
Kwon, Ki Yong;Sirowatka, Brenton;Li, Wen
通讯作者：
Li, Wen
影响因子：
4
作者：
Fekete, Z.;Csernai, M.;Bartho, P.
通讯作者：
Bartho, P.
影响因子：
4.6
作者：
Jensen, W;Yoshida, K;Hofmann, UG
通讯作者：
Hofmann, UG
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K