Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models.
Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models.
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通过体外呼吸模型的多模式成像揭示空气污染的细胞毒性作用。
DOI：
10.1098/rsos.221426
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发表时间：
2023-04
影响因子：
3.5
通讯作者：
Shaw, Michael
中科院分区：
文献类型：
--
作者：
Al-Rekabi, Zeinab;Dondi, Camilla;Faruqui, Nilofar;Siddiqui, Nazia S.;Elowsson, Linda;Rissler, Jenny;Karedal, Monica;Mudway, Ian;Larsson-Callerfelt, Anna-Karin;Shaw, Michael
据估计，每年有700万人死于接触空气中的污染物。尽管广泛的流行病学证据支持恶劣的空气质量与一系列短期和长期健康影响之间的明确联系，但在我们对污染物暴露在细胞和组织水平上引发不良生物反应的具体机制的理解上仍存在相当大的差距。更复杂、更具预测性的体外呼吸模型的发展，包括二维和三维细胞培养、球体、有机体和组织培养，以及更现实的气溶胶暴露系统，为在受控实验室条件下研究空气颗粒物的细胞毒性效应提供了新的机会。在高分辨率显微镜方面的平行进展导致了一系列体外成像工具，能够在前所未有的长度、时间和复杂性的范围内可视化和分析生物系统。本文讨论了最新的体外呼吸模型和气溶胶暴露系统，以及如何使用高分辨率显微镜技术来研究细胞与污染物的相互作用，从颗粒的摄取和运输到亚细胞细胞器和细胞的结构和功能修饰。这些数据可以提供一个机械基础，以促进我们对空气中颗粒物污染对健康影响的了解，并制定改进的缓解措施。
Annually, an estimated seven million deaths are linked to exposure to airborne pollutants. Despite extensive epidemiological evidence supporting clear associations between poor air quality and a range of short- and long-term health effects, there are considerable gaps in our understanding of the specific mechanisms by which pollutant exposure induces adverse biological responses at the cellular and tissue levels. The development of more complex, predictive, in vitro respiratory models, including two- and three-dimensional cell cultures, spheroids, organoids and tissue cultures, along with more realistic aerosol exposure systems, offers new opportunities to investigate the cytotoxic effects of airborne particulates under controlled laboratory conditions. Parallel advances in high-resolution microscopy have resulted in a range of in vitro imaging tools capable of visualizing and analysing biological systems across unprecedented scales of length, time and complexity. This article considers state-of-the-art in vitro respiratory models and aerosol exposure systems and how they can be interrogated using high-resolution microscopy techniques to investigate cell–pollutant interactions, from the uptake and trafficking of particles to structural and functional modification of subcellular organelles and cells. These data can provide a mechanistic basis from which to advance our understanding of the health effects of airborne particulate pollution and develop improved mitigation measures.
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影响因子：
3.4
作者：
Ambrose AR;Dechantsreiter S;Shah R;Montero MA;Quinn AM;Hessel EM;Beinke S;Tannahill GM;Davis DM
通讯作者：
Davis DM
影响因子：
--
作者：
Aufderheide, Michaela;Heller, Wolf-Dieter;Hochrainer, Dieter
通讯作者：
Hochrainer, Dieter
影响因子：
8.8
作者：
Bitterle, E.;Karg, E.;Hofer, T.
通讯作者：
Hofer, T.
DOI：
10.1073/pnas.1719065115
发表时间：
2018-03-13
影响因子：
11.1
作者：
Al-Rekabi, Zeinab;Contera, Sonia
通讯作者：
Contera, Sonia
DOI：
10.1007/978-1-62703-137-0_21
发表时间：
2013
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Huckabay, Heath A;Armendariz, Kevin P;Newhart, William H;Wildgen, Sarah M;Dunn, Robert C
通讯作者：
Dunn, Robert C