Aligned silk-based 3-D architectures for contact guidance in tissue engineering.
Aligned silk-based 3-D architectures for contact guidance in tissue engineering.
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DOI：
10.1016/j.actbio.2011.12.015
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发表时间：
2012-04
影响因子：
9.7
通讯作者：
Kaplan, D. L.
中科院分区：
文献类型：
--
作者：
Oliveira, A. L.;Sun, L.;Kim, H. J.;Hu, X.;Rice, W.;Kluge, J.;Reis, R. L.;Kaplan, D. L.
生物材料领域的一个重要挑战是通过细胞和细胞外基质（ECM）的排列和各向异性来模拟功能组织的结构。为了实现这一目标，使用冷冻干燥技术开发了类似骨板层结构的基于丝的支架。溶质浓度和冷冻参数可直接控制支架的结构，得到形态规则的片状支架。研究了不同的后处理，如甲醇，水退火和蒸汽灭菌，以诱导水稳定性。所得结构在形态完整性、结构和机械性能方面表现出显著差异。甲醇处理的支架的片层厚度约为2.6 μm，水退火的约为5.8 μm。这些值在人类板层骨报告的范围内。将人骨髓间充质干细胞（hMSCs）接种在这些丝素蛋白层状支架上，并在成骨条件下生长，以评估微观结构对细胞行为的影响。发现新沉积的ECM中的胶原沿着层状结构排列。在甲醇处理的层状结构的情况下，hMSC能够迁移到支架的内部，产生多层杂交构建体。本形态构成了hMSC细胞附着和增殖的有用模式，用于引导形成高度定向的细胞外基质。
An important challenge in the biomaterials field is to mimic the structure of functional tissues via cell and extracellular matrix (ECM) alignment and anisotropy. Toward this goal, silk-based scaffolds resembling bone lamellar structure were developed using a freeze-drying technique. The structure could be controlled directly by solute concentration and freezing parameters, resulting in lamellar scaffolds with regular morphology. Different post-treatments were investigated to induce water stability, such as methanol, water annealing and steam sterilization. The resulting structures exhibited significant differences in terms of morphological integrity, structure and mechanical properties. the lamellar thicknesses were around ~2,6 μm for the methanol treated scaffolds and ~5,8 μm for water-annealed. These values are in the range of the reported for human lamellar bone. Human bone marrow-derived mesenchymal stem cells (hMSCs) were seeded on these silk fibroin lamellar scaffolds and grown under osteogenic conditions to assess the effect of the microstructure on cell behaviour. Collagen in the newly deposited ECM, was found aligned along the lamellar architectures. In the case of methanol treated lamellar structures the hMSCs were able to migrate into the interior of the scaffolds producing a multilamellar hybrid construct. The present morphology constitutes a useful pattern onto which hMSCs cells attach and proliferate for guided formation of a highly oriented extracellular matrix.
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DOI：
10.1002/jbm.b.31686
发表时间：
2010-10
影响因子：
3.4
作者：
Chao, Pen-Hsiu Grace;Yodmuang, Supansa;Wang, Xiaoqin;Sun, Lin;Kaplan, David L.;Vunjak-Novakovic, Gordana
通讯作者：
Vunjak-Novakovic, Gordana
影响因子：
19
作者：
Jiang, Chaoyang;Wang, Xianyan;Tsukruk, Vladimir V.
通讯作者：
Tsukruk, Vladimir V.
影响因子：
56.9
作者：
ISRAELACHVILI, JN;MCGUIGGAN, PM
通讯作者：
MCGUIGGAN, PM
影响因子：
4.3
作者：
Enomoto, Soichiro;Sumi, Makoto;Sata, Masataka
通讯作者：
Sata, Masataka
影响因子：
14
作者：
Altman, GH;Diaz, F;Kaplan, DL
通讯作者：
Kaplan, DL