Semi-interpenetrating network (sIPN) gelatin nanofiber scaffolds for oral mucosal drug delivery.
Semi-interpenetrating network (sIPN) gelatin nanofiber scaffolds for oral mucosal drug delivery.
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DOI：
10.1016/j.actbio.2013.02.006
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发表时间：
2013-05
影响因子：
9.7
通讯作者：
Yang, Hu
中科院分区：
文献类型：
--
作者：
Aduba, Donald C., Jr.;Hammer, Jeremy A.;Yuan, Quan;Yeudall, W. Andrew;Bowlin, Gary L.;Yang, Hu
口腔粘膜是一个有前途的药物给药吸收部位，因为它是可渗透的，高度血管化，并允许易于给药。然而，用于通过口腔粘膜局部或全身药物递送的纳米纤维支架尚未被充分探索。在这项工作中，我们制备了用于口腔粘膜给药的静电纺丝明胶微球支架。为了提高静电纺丝明胶支架的结构稳定性并允许将治疗剂非侵入性地掺入支架中，我们采用光反应性聚乙二醇二丙烯酸酯（PEG-DA 575，575 gmol−1）作为交联剂，通过形成半互穿网络明胶凝胶支架（sIPN NS）来稳定支架，在此期间改变交联剂浓度（1X，2X，4X和8X）。结果表明，电纺明胶在与PEG-DA 575交联后可包覆支架（即，sIPN NS 1X、2X、4X和8X）保留了纤维形态并具有改善的结构稳定性。从形态学、纤维直径、力学性能、孔隙率、溶胀和降解等方面对交联明胶电纺支架的一系列结构参数和性能进行了系统的表征。粘蛋白在sIPN NS 4X上的吸收也得到证实，表明该支架在所测试的那些支架中具有最大的粘膜粘附特性。证明了制霉菌素（一种抗真菌试剂）从sIPN明胶支架中的缓慢释放。
The oral mucosa is a promising absorption site for drug administration because it is permeable, highly vascularized and allows for ease of administration. Nanofiber scaffolds for local or systemic drug delivery through the oral mucosa, however, have not been fully explored. In this work, we fabricated electrospun gelatin nanofiber scaffolds for oral mucosal drug delivery. To improve structural stability of the electrospun gelatin scaffolds and allow non-invasive incorporation of therapeutics into the scaffold, we employed photo-reactive polyethylene glycol diacrylate (PEG-DA575, 575 gmol−1) as a cross-linker to stabilize the scaffold by forming semi-interpenetrating network gelatin nanofiber scaffolds (sIPN NSs), during which cross-linker concentration was varied (1X, 2X, 4X, and 8X). The results showed that electrospun gelatin nanofiber scaffolds after being cross-linked with PEG-DA575 (i.e., sIPN NS1X, 2X, 4X, and 8X) retained fiber morphology and possessed improved structural stability. A series of structural parameters and properties of the cross-linked electrospun gelatin scaffolds were systematically characterized in terms of morphology, fiber diameter, mechanical properties, porosity, swelling and degradation. Mucin absorption onto sIPN NS4X was also confirmed, indicating this scaffold possessed greatest mucoadhesion properties among those tested. Slow release of nystatin, an anti-fungal reagent, from the sIPN gelatin nanofiber scaffold was demonstrated.
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