Molecular cloning and tissue expression of the fatty acid-binding protein (Es-FABP) gene in female Chinese mitten crab (Eriocheir sinensis).
Molecular cloning and tissue expression of the fatty acid-binding protein (Es-FABP) gene in female Chinese mitten crab (Eriocheir sinensis).
复制标题
雌性中华绒螯蟹脂肪酸结合蛋白（Es-FABP）基因的分子克隆及组织表达
DOI：
10.1186/1471-2199-11-71
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发表时间：
2010-09-16
影响因子：
--
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Gong YN;Li WW;Sun JL;Ren F;He L;Jiang H;Wang Q
背景脂肪酸结合蛋白（Fatty acid-binding proteins，FABP）是一种在高等脊椎动物中起重要作用的细胞质蛋白，在动物体内具有重要的生理功能。该基因全长750 bp，编码131个氨基酸，与已报道的对虾相关基因高度同源。9108 bpEs-FABP基因包含4个外显子，被3个内含子中断，这是脊椎动物FABP多基因家族成员中常见的基因组结构。RT-PCR分析显示，Es-FABP转录本在肝胰腺、血细胞、卵巢、鳃、肌肉、胸神经节、心脏和肠中均有表达，但在胃和眼柄中没有表达。实时荧光定量RT-PCR分析表明Es-FABP在卵巢、血细胞和肝胰腺中的表达依赖于卵巢的发育状态，在1月份达到高峰。间接证实了肝胰腺、卵巢和血细胞参与脂肪营养吸收和利用过程。
BackgroundFatty acid-binding proteins (FABPs), small cytosolic proteins that function in the uptake and utilization of fatty acids, have been extensively studied in higher vertebrates while invertebrates have received little attention despite similar nutritional requirements during periods of reproductive activity.ResultsTherefore, a cDNA encodingEriocheir sinensisFABP (Es-FABP) was cloned based upon EST analysis of a hepatopancreas cDNA library. The full length cDNA was 750 bp and encoded a 131 aa polypeptide that was highly homologous to related genes reported in shrimp. The 9108 bpEs-FABPgene contained four exons that were interrupted by three introns, a genomic organization common among FABP multigene family members in vertebrates. Gene expression analysis, as determined by RT-PCR, revealed the presence ofEs-FABPtranscripts in hepatopancreas, hemocytes, ovary, gills, muscle, thoracic ganglia, heart, and intestine, but not stomach or eyestalk. Real-time quantitative RT-PCR analysis revealed thatEs-FABPexpression in ovary, hemocytes, and hepatopancreas was dependent on the status of ovarian development, with peak expression observed in January.ConclusionsEvidence provided in the present report supports a role of Es-FABP in lipid transport during the period of rapid ovarian growth inE. sinensis, and indirectly confirms the participation of the hepatopancreas, ovary, and hemocytes in lipid nutrient absorption and utilization processes.
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影响因子：
2.4
作者：
MOURENTE, G;MEDINA, A;RODRIGUEZ, A
通讯作者：
RODRIGUEZ, A
影响因子：
8.8
作者：
Chen, De-Wei;Zhang, Min;Shrestha, Sundar
通讯作者：
Shrestha, Sundar
影响因子：
0.4
作者：
ALAVA, VR;KANAZAWA, A;KOSHIO, S
通讯作者：
KOSHIO, S
影响因子：
1.6
作者：
LAUTIER, J;LAGARRIGUE, JG
通讯作者：
LAGARRIGUE, JG
DOI：
10.1016/0952-3278(95)90010-1
发表时间：
1995-02-01
影响因子：
3
作者：
GLATZ, JFC;BORCHERS, T;VANDERVUSSE, GJ
通讯作者：
VANDERVUSSE, GJ