OsASR2 regulates the expression of a defence-related gene, Os2H16, by targeting the GT-1 cis-element.
OsASR2 regulates the expression of a defence-related gene, Os2H16, by targeting the GT-1 cis-element.
复制标题
DOI：
10.1111/pbi.12827
复制
发表时间：
2018-03
影响因子：
13.8
通讯作者：
Chu Z
中科院分区：
文献类型：
--
作者：
Li N;Wei S;Chen J;Yang F;Kong L;Chen C;Ding X;Chu Z
GT-1顺式元件广泛存在于多种植物基因启动子中。然而，GT-1顺式元件响应非生物和生物胁迫的分子机制在水稻中仍然是未知的。我们先前克隆了水稻短链多肽编码基因Os2H16，并证明它在抗病和抗旱中都发挥着重要的作用。在这里，我们进行了Os2H16的启动子分析，发现GT-1是一个重要的顺式元件，介导Os2H16在病原体攻击和渗透胁迫下的表达。以重复的GT-1为诱饵，从酵母一号杂交筛选中鉴定了一个脱落酸、胁迫和成熟蛋白2(ASR2)。序列比对表明，OsASR2的羧基末端结构域含有80-138个残基，是DNA结合区。此外，我们还鉴定了OsASR2与GT-1特异结合，并激活了目的基因Os2H16的表达，以及由2×10-GT-1-35S微型构建体的嵌合启动子驱动的GFP的表达。此外，在病原菌和渗透胁迫的刺激下，OsASR2的表达增加。过表达OsASR2提高了水稻对水稻白叶枯病菌的抗性。水稻的抗旱性。这些结果表明，OsASR2和GT-1之间的相互作用在水稻对生物和非生物胁迫反应的串扰中起着重要作用。
The GT‐1 cis‐element widely exists in many plant gene promoters. However, the molecular mechanism that underlies the response of the GT‐1 cis‐element to abiotic and biotic stresses remains elusive in rice. We previously isolated a rice short‐chain peptide‐encoding gene, Os2H16, and demonstrated that it plays important roles in both disease resistance and drought tolerance. Here, we conducted a promoter assay of Os2H16 and identified GT‐1 as an important cis‐element that mediates Os2H16 expression in response to pathogen attack and osmotic stress. Using the repeated GT‐1 as bait, we characterized an abscisic acid, stress and ripening 2 (ASR2) protein from yeast‐one hybridization screening. Sequence alignments showed that the carboxy‐terminal domain of OsASR2 containing residues 80–138 was the DNA‐binding domain. Furthermore, we identified that OsASR2 was specifically bound to GT‐1 and activated the expression of the target gene Os2H16, as well as GFP driven by the chimeric promoter of 2 × GT‐1‐35S mini construct. Additionally, the expression of OsASR2 was elevated by pathogens and osmotic stress challenges. Overexpression of OsASR2 enhanced the resistance against Xanthomonas oryzae pv. oryzae and Rhizoctonia solani, and tolerance to drought in rice. These results suggest that the interaction between OsASR2 and GT‐1 plays an important role in the crosstalk of the response of rice to biotic and abiotic stresses.
登录
查看更多内容
影响因子：
13.8
作者：
Helliwell, Emily E.;Wang, Qin;Yang, Yinong
通讯作者：
Yang, Yinong
影响因子：
3.5
作者：
Frankel, Nicolas;Carrari, Fernando;Iusem, Norberto D.
通讯作者：
Iusem, Norberto D.
影响因子：
1.5
作者：
Cheema, Kuljit K.;Grewal, Navjit K.;Singh, Kuldeep
通讯作者：
Singh, Kuldeep
影响因子：
6.9
作者：
Gao Y;Jia S;Wang C;Wang F;Wang F;Zhao K
通讯作者：
Zhao K
影响因子：
7.2
作者：
Kirsch, C;Logemann, E;Hahlbrock, K
通讯作者：
Hahlbrock, K