Targeting gene expression to the female larval fat body of transgenic Aedes aegypti mosquitoes.
Targeting gene expression to the female larval fat body of transgenic Aedes aegypti mosquitoes.
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DOI：
10.1111/imb.12005
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发表时间：
2013-02
影响因子：
2.6
通讯作者：
Beneš H
中科院分区：
文献类型：
--
作者：
Totten DC;Vuong M;Litvinova OV;Jinwal UK;Gulia-Nuss M;Harrell RA 2nd;Beneš H
As the fat body is a critical tissue for mosquito development, metamorphosis, immune and reproductive system function, characterization of regulatory modules targeting gene expression to the female mosquito fat body at distinct life stages is much needed for multiple, varied strategies for controlling vector-borne diseases such as dengue and malaria. The hexameric storage protein, Hexamerin-1.2, of the mosquito, Aedes atropalpus, is female-specific and uniquely expressed in the fat body of fourth-instar larvae and young adults. We have identified in the Hex-1.2 gene, a short regulatory module that directs female-, tissue-, and stage-specific lacZ reporter gene expression using a heterologous promoter in transgenic lines of the dengue vector, Aedes aegypti. Male transgenic larvae and pupae of one line expressed no E. coli β-galactosidase or transgene product; in two other lines reporter gene activity was highly female-biased. All transgenic lines expressed the reporter only in the fat body. However, lacZ mRNA levels were no different in males and females at all stages examined, suggesting that the gene regulatory module drives female-specific expression by post-transcriptional regulation in the heterologous mosquito. This regulatory element from the Hex-1.2 gene thus provides a new molecular tool for transgenic mosquito control as well as functional genetic analysis in aedine mosquitoes.
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影响因子：
3.7
作者：
Behura SK;Haugen M;Flannery E;Sarro J;Tessier CR;Severson DW;Duman-Scheel M
通讯作者：
Duman-Scheel M
影响因子：
3.8
作者：
Antonova, Yevgeniya;Alvarez, Kanwal S.;Kim, Yu Jung;Kokoza, Vladimir;Raikhel, Alexander S.
通讯作者：
Raikhel, Alexander S.
DOI：
10.1007/978-0-387-78225-6_7
发表时间：
2008-01-01
期刊：
TRANSGENESIS AND THE MANAGEMENT OF VECTOR-BORNE DISEASE
影响因子：
--
作者：
Benedict, Mark Q.;Robinson, Alan S.
通讯作者：
Robinson, Alan S.
影响因子：
64.5
作者：
BURTIS, KC;BAKER, BS
通讯作者：
BAKER, BS
DOI：
10.1073/pnas.1019295108
发表时间：
2011-03-22
影响因子：
11.1
作者：
de Valdez, Megan R. Wise;Nimmo, Derric;Black, William C.
通讯作者：
Black, William C.