Comparative genomic analysis of Drosophila melanogaster and vector mosquito developmental genes.
Comparative genomic analysis of Drosophila melanogaster and vector mosquito developmental genes.
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DOI：
10.1371/journal.pone.0021504
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Duman-Scheel M
中科院分区：
文献类型：
--
作者：
Behura SK;Haugen M;Flannery E;Sarro J;Tessier CR;Severson DW;Duman-Scheel M
基因组测序项目提供了机会，在三个媒介蚊子物种的发育基因分析：埃及伊蚊，致倦库蚊，冈比亚按蚊。本研究对黑腹果蝇和这三种重要的人类疾病载体的发育基因进行了比较基因组学分析。虽然这项研究是全面的，但特别强调的是以下基因：1）发育信号通路的组成部分，2）调节基本发育过程，3）对载体重要性组织的发育至关重要，4）已知在昆虫中分化的发育过程中的功能，以及5）编码调节果蝇发育转录物的microRNA（miRNAs）。虽然大多数果蝇发育基因在三种媒介蚊子物种中是保守的，但在一种或多种蚊子基因组中没有鉴定出已知对果蝇发育至关重要的几个基因。在其他情况下，蚊子谱系特异性基因相对于D.黑腹菌。序列分析还表明，大量重复序列是果蝇和蚊子发育基因的共同结构特征。最后，对果蝇和蚊子发育基因中预测的miRNA结合位点的分析表明，miRNA靶向的发育基因库具有物种特异性。这项研究的结果提供了深入了解双翅目和其他节肢动物的发育基因和过程的演变，作为那些追求蚊子发育分析的资源，并将促进功能分析实验的设计和完善。
Genome sequencing projects have presented the opportunity for analysis of developmental genes in three vector mosquito species: Aedes aegypti, Culex quinquefasciatus, and Anopheles gambiae. A comparative genomic analysis of developmental genes in Drosophila melanogaster and these three important vectors of human disease was performed in this investigation. While the study was comprehensive, special emphasis centered on genes that 1) are components of developmental signaling pathways, 2) regulate fundamental developmental processes, 3) are critical for the development of tissues of vector importance, 4) function in developmental processes known to have diverged within insects, and 5) encode microRNAs (miRNAs) that regulate developmental transcripts in Drosophila. While most fruit fly developmental genes are conserved in the three vector mosquito species, several genes known to be critical for Drosophila development were not identified in one or more mosquito genomes. In other cases, mosquito lineage-specific gene gains with respect to D. melanogaster were noted. Sequence analyses also revealed that numerous repetitive sequences are a common structural feature of Drosophila and mosquito developmental genes. Finally, analysis of predicted miRNA binding sites in fruit fly and mosquito developmental genes suggests that the repertoire of developmental genes targeted by miRNAs is species-specific. The results of this study provide insight into the evolution of developmental genes and processes in dipterans and other arthropods, serve as a resource for those pursuing analysis of mosquito development, and will promote the design and refinement of functional analysis experiments.
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影响因子：
11.4
作者：
Brody, T
通讯作者：
Brody, T
DOI：
10.1073/pnas.0701515104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Adelman, Zach N.;Jasinskiene, Nijole;James, Anthony A.
通讯作者：
James, Anthony A.
影响因子：
2.1
作者：
Almeras, Lionel;Fontaine, Albin;Rogier, Christophe
通讯作者：
Rogier, Christophe
影响因子：
64.5
作者：
BURTIS, KC;BAKER, BS
通讯作者：
BAKER, BS
影响因子：
3.4
作者：
Chen MS;Liu X;Yang Z;Zhao H;Shukle RH;Stuart JJ;Hulbert S
通讯作者：
Hulbert S