Identification of immune genes and proteins involved in the response of bovine mammary tissue to Staphylococcus aureus infection.
Identification of immune genes and proteins involved in the response of bovine mammary tissue to Staphylococcus aureus infection.
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DOI：
10.1186/1746-6148-4-18
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发表时间：
2008-06-02
影响因子：
2.6
通讯作者：
Tellam RL
中科院分区：
文献类型：
--
作者：
Lutzow YC;Donaldson L;Gray CP;Vuocolo T;Pearson RD;Reverter A;Byrne KA;Sheehy PA;Windon R;Tellam RL
奶牛乳房炎是由一系列微生物感染乳房组织引起的，但主要是大肠杆菌和革兰氏阳性菌，如金黄色葡萄球菌。前一种常因环境污染而获得，而S.金黄色葡萄球菌由于其对抗生素治疗的抗性和以慢性亚临床状态驻留在乳腺组织内的能力而特别成问题。牛乳腺上皮组织内S.金黄色葡萄球菌的特征很差，特别是在感染的最早阶段。此外，感染对乳汁中存在的生物活性先天免疫蛋白的影响也不清楚。这些反应的性质可以决定组织的易感性及其解决感染的能力。采用转录谱分析方法，测定了三头奶牛在用沙门氏菌进行短暂和分级的乳房内攻击后，乳房组织中基因表达的变化。金黄色。这些有限的挑战对编码β-酪蛋白的基因的表达模式没有显著影响，但引起参与促炎反应的许多细胞因子和趋化因子的协调上调。此外，S100钙结合蛋白A12（S100 A12）和Pentraxin-3（PTX 3）这两个基因的表达增强与感染乳房的乳汁中蛋白质水平的显著增加相对应。这两个基因被证明是表达的乳腺上皮细胞培养物中生长的刺激后，脂多糖。体细胞计数（一种广泛使用的乳腺炎指标）与一群奶牛乳汁中的S100 A12水平之间也存在很强的相关性。重组S100 A12在体外抑制大肠杆菌的生长，重组PTX 3与大肠杆菌结合。大肠杆菌以及C1 q，补体级联反应的第一组分的亚基。即使在低剂量的细菌和短时间的感染，在受感染的牛乳腺组织的转录反应，可能反映了乳腺上皮细胞和浸润免疫细胞的激活导致的基因表达变化的综合贡献。由细菌刺激的乳腺上皮细胞分泌的许多促炎细胞因子和趋化因子用于触发乳腺组织中中性粒细胞的募集和活化。来自受感染乳房区的乳汁中存在S100 A12和PTX 3可能会增加乳汁的抗菌特性，从而有助于解决乳腺组织感染，并可能有助于新生小牛上皮细胞的成熟和新生儿肠道微生物群的建立。
Mastitis in dairy cattle results from infection of mammary tissue by a range of micro-organisms but principally coliform bacteria and Gram positive bacteria such as Staphylococcus aureus. The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. The transcriptional responses within bovine mammary epithelial tissue subjected to intramammary challenge with S. aureus are poorly characterised, particularly at the earliest stages of infection. Moreover, the effect of infection on the presence of bioactive innate immune proteins in milk is also unclear. The nature of these responses may determine the susceptibility of the tissue and its ability to resolve the infection. Transcriptional profiling was employed to measure changes in gene expression occurring in bovine mammary tissues sampled from three dairy cows after brief and graded intramammary challenges with S. aureus. These limited challenges had no significant effect on the expression pattern of the gene encoding β-casein but caused coordinated up-regulation of a number of cytokines and chemokines involved in pro-inflammatory responses. In addition, the enhanced expression of two genes, S100 calcium-binding protein A12 (S100A12) and Pentraxin-3 (PTX3) corresponded with significantly increased levels of their proteins in milk from infected udders. Both genes were shown to be expressed by mammary epithelial cells grown in culture after stimulation with lipopolysaccharide. There was also a strong correlation between somatic cell count, a widely used measure of mastitis, and the level of S100A12 in milk from a herd of dairy cows. Recombinant S100A12 inhibited growth of Escherichia coli in vitro and recombinant PTX3 bound to E. coli as well as C1q, a subunit of the first component of the complement cascade. The transcriptional responses in infected bovine mammary tissue, even at low doses of bacteria and short periods of infection, probably reflect the combined contributions of gene expression changes resulting from the activation of mammary epithelial cells and infiltrating immune cells. The secretion of a number of proinflammatory cytokines and chemokines from mammary epithelial cells stimulated by the bacteria serves to trigger the recruitment and activation of neutrophils in mammary tissue. The presence of S100A12 and PTX3 in milk from infected udder quarters may increase the anti-bacterial properties of milk thereby helping to resolve the mammary tissue infection as well as potentially contributing to the maturation of the newborn calf epithelium and establishment of the newborn gut microbial population.
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DOI：
10.1046/j.1439-0450.2003.00628.x
发表时间：
2003-04-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Alluwaimi, AM;Leutenegger, CM;Cullor, JS
通讯作者：
Cullor, JS
影响因子：
3.3
作者：
Feng, W;Zheng, XD
通讯作者：
Zheng, XD
影响因子：
3.5
作者：
De Oliveira, AP;Watts, JL;Aerestrup, FM
通讯作者：
Aerestrup, FM
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.4
作者：
Bannerman, DD;Paape, MJ;Hope, JC
通讯作者：
Hope, JC