B-cell diversity decreases in old age and is correlated with poor health status.
B-cell diversity decreases in old age and is correlated with poor health status.
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DOI：
10.1111/j.1474-9726.2008.00443.x
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发表时间：
2009-02
期刊：
影响因子：
7.8
通讯作者：
Dunn-Walters DK
中科院分区：
文献类型：
--
作者：
Gibson KL;Wu YC;Barnett Y;Duggan O;Vaughan R;Kondeatis E;Nilsson BO;Wikby A;Kipling D;Dunn-Walters DK
老年人的免疫系统下降，影响他们对感染作出反应和对疫苗作出有效反应的能力。有效和特异性抗体的反应，从老年人减少有利于非特异性抗体的生产。我们研究了年龄在86-94岁的个体和年龄在19-54岁的对照组外周血DNA样本中的B细胞库，使用IGHV互补决定区（CDR）3的谱型分析。我们发现，一部分老年人的B细胞多样性急剧下降。从选择的样品的聚合酶链反应产物的测序表明，这种多样性的损失的特点是在体内的B细胞的克隆扩增。光谱的统计分析使客观的比较，并表明，损失的多样性与个人的一般健康状况非常密切相关;扭曲的光谱型可以用来预测脆弱。还观察到与生存和维生素B12状态的相关性。我们的结论是，B细胞多样性会随着年龄的增长而急剧下降，可能对老年人的免疫健康产生重要影响。B细胞免疫力低下也是全身虚弱的标志。
Older people suffer from a decline in immune system, which affects their ability to respond to infections and to raise efficient responses to vaccines. Effective and specific antibodies in responses from older individuals are decreased in favour of non-specific antibody production. We investigated the B-cell repertoire in DNA samples from peripheral blood of individuals aged 86–94 years, and a control group aged 19–54 years, using spectratype analysis of the IGHV complementarity determining region (CDR)3. We found that a proportion of older individuals had a dramatic collapse in their B-cell repertoire diversity. Sequencing of polymerase chain reaction products from a selection of samples indicated that this loss of diversity was characterized by clonal expansions of B cells in vivo. Statistical analysis of the spectratypes enabled objective comparisons and showed that loss of diversity correlated very strongly with the general health status of the individuals; a distorted spectratype can be used to predict frailty. Correlations with survival and vitamin B12 status were also seen. We conclude that B-cell diversity can decrease dramatically with age and may have important implications for the immune health of older people. B-cell immune frailty is also a marker of general frailty.
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影响因子：
2.6
作者：
Dace, Dru S.;Apte, Rajendra S.
通讯作者：
Apte, Rajendra S.
DOI：
10.1111/j.1365-2257.2006.00755.x
发表时间：
2006-02-01
期刊：
CLINICAL AND LABORATORY HAEMATOLOGY
影响因子：
--
作者：
Andrès, E;Affenberger, S;Blicklé, JF
通讯作者：
Blicklé, JF
影响因子：
5.3
作者：
Banerjee, M;Sanderson, JD;Dunn-Walters, DK
通讯作者：
Dunn-Walters, DK
影响因子：
2.6
作者：
Colonna-Romano, G;Aquino, A;Caruso, C
通讯作者：
Caruso, C
影响因子：
5.4
作者：
DunnWalters, DK;Boursier, L;Spencer, J
通讯作者：
Spencer, J