Functional Characterization of Replication-Associated Proteins Encoded by Alphasatellites Identified in Yunnan Province, China.
Functional Characterization of Replication-Associated Proteins Encoded by Alphasatellites Identified in Yunnan Province, China.
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中国云南省发现的α卫星编码的复制相关蛋白的功能表征
DOI：
10.3390/v14020222
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发表时间：
2022-01-24
期刊：
影响因子：
--
通讯作者：
Xie Y
中科院分区：
文献类型：
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作者：
Zhao L;Che X;Wang Z;Zhou X;Xie Y
α卫星仅编码一种复制相关蛋白（α - rep），经常被发现是与begomavirus / β卫星复合物相关的非必需卫星成分，它们的存在可在感染期间调节疾病症状和/或病毒DNA积累。我们之前的研究表明，在中国云南省存在三种与begomvirus /betasatellite复合物相关的α -卫星，它们编码三种相应的α - rep蛋白。然而，α - reps的生物学功能仍然知之甚少。在本研究中，我们以16c和16-TGS转基因本烟植物为研究对象，研究了α - reps在转录后基因沉默（PTGS）和转录基因沉默（TGS）中的生物学功能。结果表明，3种α - rep蛋白均能抑制PTGS并逆转TGS。其中，Y10DNA1的α - rep具有最强的PTGS和TGS抑制活性。我们还发现，在合并感染期间，α - rep蛋白能够增加其辅助病毒的积累。这些结果表明，α - rep可能在克服宿主防御中起作用，这可能解释了α -卫星与begomvirus / β -卫星复合物相关联所提供的选择优势。
Alphasatellites, which encode only a replication-associated protein (alpha-Rep), are frequently found to be non-essential satellite components associated with begomovirus/betasatellite complexes, and their presence can modulate disease symptoms and/or viral DNA accumulation during infection. Our previous study has shown that there are three types of alphasatellites associated with begomovirus/betasatellite complexes in Yunnan province in China and they encode three corresponding types of alpha-Rep proteins. However, the biological functions of alpha-Reps remain poorly understood. In this study, we investigated the biological functions of alpha-Reps in post-transcriptional gene silencing (PTGS) and transcriptional gene silencing (TGS) using 16c and 16-TGS transgenic Nicotiana benthamiana plants. Results showed that all the three types of alpha-Rep proteins were capable of suppressing the PTGS and reversing the TGS. Among them, the alpha-Rep of Y10DNA1 has the strongest PTGS and TGS suppressor activities. We also found that the alpha-Rep proteins were able to increase the accumulation of their helper virus during coinfection. These results suggest that the alpha-Reps may have a role in overcoming host defense, which provides a possible explanation for the selective advantage provided by the association of alphasatellites with begomovirus/betasatellite complexes.
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影响因子：
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作者：
Abbas, Qamar;Amin, Imran;Briddon, Rob W.
通讯作者：
Briddon, Rob W.
影响因子：
5.4
作者：
Cui, XF;Tao, XR;Zhou, XP
通讯作者：
Zhou, XP
DOI：
10.1073/pnas.182204199
发表时间：
2002-09-03
影响因子：
11.1
作者：
Klahre, U;Crété, P;Meins, F
通讯作者：
Meins, F
影响因子：
5.4
作者：
Martinez, Fernando;Daros, Jose-Antonio
通讯作者：
Daros, Jose-Antonio
DOI：
10.1038/nrg2719
发表时间：
2010-03
期刊：
Nature reviews. Genetics
影响因子：
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作者：
通讯作者：
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