Dual peptides modified fluorescence-SERS dual mode imaging nanoprobes with improved cancer cell targeting efficiency
Dual peptides modified fluorescence-SERS dual mode imaging nanoprobes with improved cancer cell targeting efficiency
复制标题
双肽修饰荧光-SERS双模式成像纳米探针提高癌细胞靶向效率
DOI：
10.1039/c6ra13802k
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发表时间：
2016-08
期刊：
影响因子：
3.9
通讯作者：
Yiping Cui
中科院分区：
文献类型：
--
作者：
Yizhi Zhang;Zhuyuan Wang;Lei Wu;Shenfei Zong;Binfeng Yun;Yiping Cui
癌症治疗学领域长期以来一直渴望具有改进的靶向效率的成像剂。本文中，具有荧光-SERS双模式成像能力的双受体靶向纳米探针已被证明用于特异性靶向宫颈癌细胞。首先，银纳米粒子修饰与拉曼报告和二氧化硅保护的荧光染料，以纳入荧光-SERS双模成像能力。然后，两个靶向肽，转铁蛋白受体特异性肽T7和整合素ανβ3结合肽RGD，被功能化到纳米颗粒的外表面上，赋予纳米探针对HeLa细胞的增强识别。最后，为了研究这种协同探针的靶向能力，比较了单靶向和双靶向纳米探针之间的细胞内化。荧光-SERS双模式成像的结果表明，双肽功能化纳米探针不仅促进了癌细胞的摄取，而且还增强了对癌细胞的靶向能力。这种具有双模式成像和双靶向能力的纳米探针将为癌症的诊断和治疗提供新的前景。
The field of cancer theragnostics has long been starving for imaging agents with an improved targeting efficiency. Herein, a dual receptor targeting nanoprobe with fluorescence-SERS dual mode imaging capacity has been demonstrated for the specific targeting of cervical cancer cells. First, silver nanoparticles were modified with Raman reporters and silica-protected fluorescence dyes to incorporate fluorescence-SERS dual mode imaging capacity. Then, two targeting peptides, transferrin receptor-specific peptide T7 and integrin ανβ3 bonding peptide RGD, were functionalized onto the outer surfaces of the nanoparticles, endowing the nanoprobes with enhanced recognition towards HeLa cells. Finally, to investigate the targeting capability of this synergetic probe, the cellular internalization was compared between single and dual targeting nanoprobes. The results from fluorescence-SERS dual-mode imaging demonstrated that the dual peptide functionalized nanoprobes not only promoted an elevated uptake by cancer cells, but were also responsible for an enhanced targeting ability towards cancer cells. This type of nanoprobe with dual-mode imaging and a dual-targeting ability would give a new prospect for the diagnosis and therapeutics of cancer.
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