Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines.
Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines.
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DOI：
10.1007/s11262-012-0776-z
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发表时间：
2012-10
期刊：
影响因子：
1.6
通讯作者：
Oshitani, Hitoshi
中科院分区：
文献类型：
--
作者：
Apostol, Lea Necitas G.;Imagawa, Tomifumi;Suzuki, Akira;Masago, Yoshifumi;Lupisan, Socorro;Olveda, Remigio;Saito, Mariko;Omura, Tatsuo;Oshitani, Hitoshi
关键词：
尽管全球报告的肠道病毒分布广泛且多样性广泛，但尚未建立定义菲律宾等热带国家肠道病毒流行病学完整观点的指标。环境中肠道病毒的检测已成为社区流行病毒的标志之一。本研究旨在通过概述菲律宾城市和农村河流中肠道病毒的发生情况，弥补菲律宾肠道病毒流行病学的差距。对2009年4月至12月采集的44份抓斗河水样品进行了针对肠道病毒基因组VP 1区域的分子检测。在42例肠道病毒血清型中，以人肠道病毒C型（HEV-C; 21/42）为主，其次为HEV-B型（12/42）和HEV-A型（9/42）。在44份水样中，有12份发现了猪肠道病毒9型。系统发育分析表明，检测到的病毒与以前从该国急性弛缓性麻痹病例中检测到的肠道病毒密切相关，如果不是全部形成单系分支的话。环境和人类肠道病毒株的聚类表明，这些菌株的循环与河流污染有关。这项研究进一步证明了肠道病毒在粪便中传播后的环境持久性，同样，提供了额外的数据，可能有助于了解肠道病毒在人类中的流行病学，强调需要更多的研究与环境中发现的肠道病毒及其最终在该国的临床后果有关的潜在公共卫生风险。本文的在线版本（doi：10.1007/s11262-012-0776-z）包含补充材料，可供授权用户使用。
Despite the vast distribution and expansive diversity of enteroviruses reported globally, indicators defining a complete view of the epidemiology of enteroviruses in tropical countries such as the Philippines are yet to be established. Detection of enteroviruses in the environment has been one of the markers of circulating viruses in a community. This study aimed to bridge the gap in the epidemiology of enteroviruses in the Philippines by providing an overview of the occurrence of enteroviruses in both urban and rural rivers. Molecular detection directed at the VP1 region of the enterovirus genome was performed on 44 grab river water samples collected from April to December 2009. The majority of the enterovirus serotypes detected were clustered with human enterovirus C species (HEV-C; 21/42), followed by HEV-B (12/42) and HEV-A (9/42). Porcine enterovirus 9 was also found in 12 out of 44 water samples. Phylogenetic analysis indicated that the viruses detected were closely related, if not all forming a monophyletic clade, with those enteroviruses detected previously from acute flaccid paralysis cases in the country. The clustering of environmental and human enterovirus strains implies that the circulation of these strains were associated with river contamination. This study gives further evidence of the environmental persistence of enteroviruses once they are shed in feces and likewise, provides additional data which may help in understanding the epidemiology of enteroviruses in humans, highlighting the need for more studies on the potential public health risks linked with enteroviruses found in the environment and their eventual clinical consequences in the country. The online version of this article (doi:10.1007/s11262-012-0776-z) contains supplementary material, which is available to authorized users.
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